Anti-MECP2 Polyclonal Antibody for WB, IF/ICC, ELISA - P51608

Core Product Specifications and Parameters

Biological Background: MECP2 Function and Localization

• Encodes Methyl-CpG-binding protein 2, a chromosomal protein essential for reading DNA methylation marks and regulating transcription.
• Binds specifically to single methylated CpG pairs, mediating transcriptional repression via recruitment of histone deacetylase and the SIN3A corepressor complex.
• Recognizes both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) with higher affinity for 5mC, linking DNA methylation to gene silencing.
• Localizes predominantly to the nucleus, consistent with its role in chromatin interaction and epigenetic regulation.
• Ubiquitously expressed in adult somatic tissues, highlighting its fundamental function in cellular epigenetic maintenance.
• Mutations in MECP2 are causal for Rett syndrome (RTT) and associated with autism spectrum disorders (AUTSX3) and X-linked intellectual disability (MRXS13).
• Subject to post-translational modifications including acetylation, phosphorylation, and methylation, which may modulate its DNA-binding and repressor activities.

Experimental Guidance and Technical Tips

• The immunogen was raised against a synthetic peptide spanning amino acids 387–486 in the C-terminal region of human MECP2. Cross-reactivity with mouse and rat is predicted due to sequence conservation, but validation in the target species is advised.
• For Western blotting, expect a band at approximately 52 kDa. Use nuclear-enriched lysates from human, mouse, or rat cells/tissues as positive controls and confirm specificity.
• In immunofluorescence/ICC experiments, MECP2 shows nuclear staining. Optimize permeabilization and fixation protocols for nuclear antigens to ensure accurate localization.
• For ELISA applications, the antibody can detect MECP2 peptides or recombinant protein. Standard direct or indirect ELISA protocols are suitable; titrate antibody concentration for optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials for epigenetic and neurodevelopmental research. As your sourcing partner, we supply validated antibody pairs, optimized polyclonal antibodies like this anti-MECP2 product, and bulk ancillary reagents to accelerate your assay development. Whether you need rare target raw-material sourcing or scalable production, CamelBio bridges your concept to clinic with reliable supply solutions.