Anti-MECP2 Polyclonal Antibody for WB, ELISA - P51608

Core Product Specifications and Parameters

Biological Background: MECP2 Function and Localization

• MECP2 (Methyl-CpG-binding protein 2) is a chromosomal protein that specifically binds methylated DNA, playing a crucial role in transcriptional regulation and chromatin architecture.
• It mediates transcriptional repression by recruiting histone deacetylase and the corepressor SIN3A to methylated promoter regions.
• MECP2 binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a higher affinity for 5mC, linking DNA methylation to gene silencing.
• The protein is predominantly localized to the nucleus, consistent with its function as a DNA-binding transcriptional repressor.
• MECP2 is expressed ubiquitously in all adult somatic tissues, underscoring its fundamental regulatory role across diverse cell types.
• Post-translational modifications (acetylation, phosphorylation) and alternative splicing contribute to the functional diversity of MECP2.
• Mutations in MECP2 are associated with neurodevelopmental disorders, most notably Rett syndrome (RTT), as well as autism spectrum disorders and intellectual disabilities.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to the C-terminal region (amino acids 387-486) of human MECP2, which includes domains important for protein interactions; consider this when designing epitope-tagging or competitive assays.
• For Western blot (WB), MECP2 is detected at approximately 52kDa; ensure thorough nuclear protein extraction to enrich the target.
• For ELISA applications, validate the antibody against recombinant or endogenous MECP2 in the relevant sample system, and consider using methylated DNA oligonucleotides for functional binding assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials for diagnostic manufacturers and research laboratories, including validated antibodies and ancillary reagents. This anti-MECP2 polyclonal antibody is part of our range tailored for epigenetics and neurodevelopmental disorder research. Leverage our procurement expertise to source paired antibodies, bulk reagents, and custom production support across your clinical and translational projects.