Anti-MECOM Rabbit Polyclonal Antibody for WB, ELISA - Q03112 / Q13465

Core Product Specifications and Parameters

Biological Background: MECOM Function and Localization

• MECOM (EVI1, MDS1, PRDM3) is a transcriptional regulator and histone-lysine N-methyltransferase that can activate or repress gene expression.
• It plays an oncogenic role in development, cell proliferation, differentiation, and apoptosis, with involvement in hematopoiesis.
• MECOM catalyzes monomethylation of histone H3 at Lys-9 (H3K9me1) in the cytoplasm, a critical step for subsequent chromatin methylation in the nucleus.
• The protein is primarily localized in the nucleus and nuclear speckles, but also detectable in the cytoplasm.
• Contains multiple zinc finger domains and is subject to acetylation, phosphorylation, and ubiquitination.
• Alternative splicing and promoter usage produce multiple isoforms with molecular weights ranging from 18 to 139 kDa.
• Mutations in MECOM are associated with myelodysplastic syndromes and acute myeloid leukemia.
• Keywords: DNA-binding, methyltransferase, proto-oncogene, zinc-finger, alternative splicing, disease variant.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–190 of human MECOM (NP_004982.2), targeting the N-terminal region shared by several isoforms. Consider this when interpreting bands of different molecular weights (18, 117, 118, 125, 139 kDa) in Western blot.
• For ELISA, use recombinantly expressed MECOM fragments to verify antibody specificity.
• Validate the antibody in relevant human or rat cell lines known to express MECOM, such as hematopoietic cells or cancer lines with MECOM overexpression.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibodies, recombinant proteins, and ancillary reagents, to support diagnostic development from research to clinic. This anti-MECOM polyclonal antibody is suitable for studying transcriptional regulation, hematopoiesis, and leukemia-associated pathways. Whether you need bulk quantities, customized antibody pairs, or rare target sourcing, CamelBio offers reliable one-stop procurement solutions to accelerate your project.