Anti-MEAF6 Polyclonal Antibody for WB, ELISA - Q9HAF1

Core Product Specifications and Parameters

Biological Background: MEAF6 Function and Localization

• MEAF6, also known as CENP-28, EAF6, and NY-SAR-91, is a chromatin modification-related protein and a core component of several histone acetyltransferase complexes.
• It is a component of the NuA4 histone acetyltransferase complex, which acetylates histone H4 and H2A, thereby modulating chromatin structure and promoting transcriptional activation. Related references: PMID:14966270
• MEAF6 is also a subunit of the HBO1 complex, which specifically mediates acetylation of histone H3 at Lys-14 (H3K14ac), with reduced activity toward histone H4. Related references: PMID:16387653, PMID:24065767
• It participates in the MOZ/MORF complex, which possesses histone H3 acetyltransferase activity and contributes to gene regulation. Related references: PMID:18794358
• Subcellularly, MEAF6 localizes to the nucleus and nucleolus, and is specifically found at the centromere and kinetochore, suggesting a role in chromosome segregation.
• The protein is subject to post-translational modifications including phosphorylation and ubiquitination (isopeptide bond), which may regulate its activity and stability.
• Alternative splicing and chromosomal rearrangements involving MEAF6 have been implicated in certain cancers; the NY-SAR-91 alias reflects its identification as a sarcoma antigen.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was generated using a recombinant immunogen spanning amino acids 1-191 of human MEAF6. It is recommended for use in Western blot (WB) and ELISA applications.
• For WB, consider using standard reducing conditions and optimizing primary antibody dilution in the range of 1:500-1:2000. Validate in appropriate human or mouse cell lines known to express MEAF6, such as HeLa or NIH/3T3.
• Due to the protein's predicted molecular weight of 22 kDa, ensure adequate separation in SDS-PAGE using 12-15% gels and verify species cross-reactivity carefully.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio serves as a comprehensive supply partner for IVD raw materials, offering an extensive portfolio of validated antibodies, antigens, and ancillary reagents. This MEAF6 polyclonal antibody supports research and diagnostic development in epigenetics, chromatin biology, and cancer biomarker discovery. Our one-stop sourcing model simplifies procurement, providing consistent batch-to-batch quality, technical consultation, and efficient logistics from concept to clinic.