Anti-ME3 (NADP-ME) Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q16798

Core Product Specifications and Parameters

Biological Background: ME3 Function and Localization

• ME3 (Malic enzyme 3) is a mitochondrial NADP-dependent malic enzyme that catalyzes the oxidative decarboxylation of (S)-malate to pyruvate and CO₂, with NADP+ as cofactor. Related references: PMID:7818469
• It can also catalyze the decarboxylation of oxaloacetate into pyruvate, though with lower efficiency.
• The reverse reaction (reductive carboxylation of pyruvate to malate) is possible but occurs with significantly lower efficiency.
• ME3 localizes to the mitochondrial matrix, where it participates in central carbon metabolism.
• The protein undergoes alternative splicing, producing multiple isoforms, and is regulated by phosphorylation (Phosphoprotein keyword).
• It is expressed predominantly in organs with low cell division rates, suggesting a role in tissues with reduced proliferative activity.
• Keywords include 3D-structure, metal-binding, NADP, oxidoreductase, and reference proteome, indicating its well-characterized enzymatic function.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein corresponding to amino acids 505–604 near the C-terminus of human ME3; this region is expected to be accessible in both native and denatured protein conformations.
• For Western blot (WB), the predicted molecular weight of ME3 is approximately 67 kDa; validate using a positive control lysate from human, mouse, or rat tissues known to express ME3.
• In immunohistochemistry (IHC-P), consider using antigen retrieval methods and tissues with low proliferation rates, as ME3 expression is enriched in such organs.
• For immunofluorescence (IF/ICC), mitochondrial staining may require permeabilization; co-localization with mitochondrial markers can confirm specificity.
• When using ELISA, the antibody may be employed as a capture or detection reagent; pilot experiments to optimize coating concentration and blocking conditions are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs and bulk ancillary reagents, supporting manufacturers and labs from concept to clinic. The ME3 polyclonal antibody is part of our metabolic enzyme antibody collection, suitable for research in mitochondrial disorders and cancer metabolism. Contact us for sourcing customized antibodies or rare target reagents to accelerate your assay development.