Anti-MDM2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q00987

Core Product Specifications and Parameters

Biological Background: MDM2 Function and Localization

• MDM2 (also known as Hdm2, HDMX) is an E3 ubiquitin-protein ligase that serves as a critical negative regulator of the tumor suppressor p53/TP53, mediating its ubiquitination and proteasomal degradation. Related references: PMID:29681526
• It inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding to their transcriptional activation domains, and promotes nuclear export of p53.
• MDM2 also acts as a ubiquitin ligase toward itself (auto-ubiquitination) and other substrates including ARRB1, RB1, DAXX, DYRK2, IGF1R, SNAI1, DCX, DLG4, and NDUFS1, regulating diverse cellular processes such as receptor endocytosis, mitochondrial respiration, and apoptosis. Related references: PMID:12821780, PMID:15053880, PMID:15195100, PMID:30879903
• The protein localizes predominantly to the nucleus, including the nucleoplasm and nucleolus, but can also be found in the cytoplasm, reflecting its dynamic roles in different compartments.
• MDM2 is ubiquitously expressed in normal tissues, while several cancer-associated isoforms (Mdm2-A through Mdm2-G) are observed in a range of cancers but absent in normal tissues.
• It contains a RING-type zinc finger domain and is a phosphoprotein, with post-translational modifications influencing its activity and stability.
• Due to its oncogenic properties, MDM2 is classified as a proto-oncogene and is involved in apoptosis, host-virus interaction, and the ubiquitin conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide corresponding to an internal region (amino acids 231-330) of human MDM2. This design may enable recognition of multiple isoforms and processed forms of MDM2.
• For Western blotting (WB), a band at approximately 55 kDa is expected; consider validating specificity using positive and negative cell lysates.
• For immunohistochemistry (IHC-P) and ELISA, optimize antibody dilution and detection conditions, as staining patterns may vary with tissue type and fixation methods.
• Given the sequence homology, cross-reactivity with mouse MDM2 is anticipated; validate in relevant mouse model systems if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for high-quality IVD raw materials, dedicated to supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. This anti-MDM2 polyclonal antibody is part of our extensive portfolio of cancer research-associated reagents. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development.