Anti-MDM2 Rabbit Polyclonal Antibody for WB and ELISA - Q00987

Core Product Specifications and Parameters

Biological Background: MDM2 Function and Localization

• MDM2 is an E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its proteasomal degradation. It inhibits p53- and p73-mediated cell cycle arrest and apoptosis by directly binding their transcriptional activation domains. Related references: PMID:29681526
• MDM2 also acts as an E3 ubiquitin ligase toward itself and ARRB1, permits the nuclear export of p53, promotes ubiquitin-independent degradation of RB1, and inhibits DAXX-mediated apoptosis via ubiquitination and degradation.
• MDM2 ubiquitinates IGF1R and SNAI1, targeting them for proteasome-dependent degradation. Related references: PMID:12821780 PMID:15053880 PMID:15195100
• MDM2 negatively regulates mitochondrial respiration by binding NDUFS1, causing its cytosolic retention, reduced complex I activity, increased ROS production, and commitment to apoptosis. Related references: PMID:30879903
• The protein localizes to the nucleus (nucleoplasm and nucleolus) and cytoplasm, consistent with its roles in transcriptional regulation and cytoplasmic signaling.
• MDM2 is ubiquitously expressed, but several cancer-associated isoforms (Mdm2-A through Mdm2-G) are detected in tumors and absent in normal tissues.
• Classified as a proto-oncogene, MDM2 is a phosphoprotein with a RING-type zinc finger domain and participates in host-virus interactions and the ubiquitin conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 200–430 of human MDM2, covering the acidic domain, zinc finger, and RING finger regions conserved across human, mouse, and rat. For Western blotting, consider using lysates from MDM2-overexpressing cancer cell lines (e.g., SJSA-1) as positive controls, and validate detection in mouse and rat tissues with appropriate species-specific secondary antibodies.
• A primary band of ~55 kDa is expected in WB; higher molecular weight species may represent post-translationally modified forms (e.g., ubiquitinated or phosphorylated MDM2). Including phosphatase inhibitors during sample preparation may help preserve the expected molecular weight.
• For ELISA, test the antibody in both direct antigen coating and sandwich formats. Preliminary checkerboard titrations are recommended to optimize coating antigen and antibody working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with a one-stop sourcing solution for premium IVD raw materials, including validated antibody pairs, purified antigens, and bulk ancillary reagents. This anti-MDM2 antibody supports research into the p53 tumor suppressor pathway and cancer biology. Beyond individual antibodies, CamelBio offers tailored sourcing and technical consulting to streamline your assay development from concept to clinic.