Anti-MDM2 Rabbit Monoclonal Antibody for WB, ELISA - Q00987

Core Product Specifications and Parameters

Biological Background: MDM2 Function and Localization

• MDM2, also known as Hdm2, is an E3 ubiquitin-protein ligase that catalyzes the ubiquitination of p53/TP53, targeting it for proteasomal degradation (PubMed:29681526). Related references: PMID:29681526

• As an oncoprotein, MDM2 inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding their transcriptional activation domains.

• The protein also ubiquitinates itself and ARRB1, and mediates proteasome-dependent degradation of RB1 protein, thereby modulating cell cycle progression.

• It promotes nuclear export of p53/TP53 and ubiquitinates various substrates including IGF1R and SNAI1 for proteasomal degradation (PubMed:12821780, PubMed:15053880, PubMed:15195100). Related references: PMID:12821780, PMID:15053880, PMID:15195100

• MDM2 negatively regulates mitochondrial respiration by binding NDUFS1, causing its cytosolic retention and leading to mitochondrial dysfunction and apoptosis (PubMed:30879903). Related references: PMID:30879903

• Subcellularly, MDM2 localizes to the nucleus, nucleoplasm, cytoplasm, and nucleolus, consistent with its roles in protein degradation and nucleocytoplasmic shuttling.

• Although MDM2 is ubiquitously expressed in normal tissues, cancer-specific isoforms (Mdm2-A through Mdm2-G) have been identified, underscoring its relevance in oncogenesis.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 101-200 of human MDM2 (NP_001354919.1). This region may be conserved across isoforms; verify expected reactivity with specific variants.
• For Western blot, consider using positive-control cell lines expressing MDM2 and validate the observed band(s) against the reported complex pattern of protein weights (ranging from 11 kDa to 55 kDa).
• For ELISA, standardize with recombinant MDM2 protein or characterized cell lysates; titrate antibody concentration to achieve optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop IVD raw-material sourcing partner, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-MDM2 rabbit monoclonal antibody supports research in oncology, protein ubiquitination, and apoptosis, while CamelBio’s supply capabilities can also assist in sourcing rare-target raw materials for your diagnostic development needs.