Anti-MDM2 Rabbit Monoclonal Antibody for IHC-P and ELISA - Q00987

Core Product Specifications and Parameters

Biological Background: MDM2 Function and Localization

• MDM2 is an E3 ubiquitin ligase that mediates ubiquitination of p53/TP53, targeting it for proteasomal degradation (PubMed:29681526). Related references: PMID:29681526
• It inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis, and also ubiquitinates itself and ARRB1.
• MDM2 permits nuclear export of p53/TP53 and promotes degradation of retinoblastoma protein RB1 and DAXX.
• It ubiquitinates and promotes proteasomal degradation of IGF1R and SNAI1 (PubMed:12821780, PubMed:15053880, PubMed:15195100). Related references: PMID:12821780, PMID:15053880, PMID:15195100
• MDM2 negatively regulates mitochondrial respiration by binding NDUFS1, causing oxidative stress and apoptosis (PubMed:30879903). Related references: PMID:30879903
• Subcellular localization is predominantly nuclear (nucleoplasm, nucleolus) and cytoplasmic.
• MDM2 is ubiquitously expressed; specific isoforms (A–G) are detected in various cancers but absent in normal tissues.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 101–200; consider verifying peptide blocking experiments in peptide-based ELISA setups.
• For IHC-P, optimize antigen retrieval and primary antibody dilution; include positive and negative tissue controls.
• This rabbit monoclonal antibody ensures batch-to-batch consistency; validate in endogenously MDM2-expressing human cell lines or tumor tissues.
• Given human-specific cross reactivity, confirm reactivity if working with non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports IVD manufacturers and research laboratories with comprehensive raw-material procurement for cancer and ubiquitination research. In addition to this anti-MDM2 rabbit monoclonal antibody, we supply validated antibody pairs, bulk ancillary reagents, and can facilitate rare-target sourcing. Contact us to streamline your project from concept to clinic.