Anti-MDH2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P40926

Core Product Specifications and Parameters

Biological Background: MDH2 Function and Localization

• MDH2 is the mitochondrial isoform of malate dehydrogenase, encoded by the MDH2 gene (GeneID: 4191), with a molecular weight of approximately 36 kDa.
• It catalyzes the reversible oxidation of malate to oxaloacetate using NAD+ as a cofactor, a key step in the tricarboxylic acid (TCA) cycle for cellular respiration and energy production. Related references: PMID:27989324 PMID:35366151
• MDH2 is central to the malate-aspartate shuttle, transferring reducing equivalents across the mitochondrial membrane to maintain NAD+/NADH homeostasis (By similarity).
• The enzyme localizes to the mitochondrial matrix, where it functions in TCA cycle and redox balance.
• MDH2 undergoes various post-translational modifications, including acetylation, phosphorylation, and palmitoylation, which may regulate its activity or localization.
• Mutations in MDH2 are associated with developmental and epileptic encephalopathy (DEE51/EIEE51), highlighting its importance in neuronal metabolism.
• Recent studies show MDH2 can bind RNA, including cytosolic tRNAs and mRNAs, suggesting non-metabolic roles. Related references: PMID:38609156

Experimental Guidance and Technical Tips

• For Western blot (WB), the expected band is around 36 kDa. Consider using appropriate positive controls (e.g., mitochondrial lysates) and validate antibody specificity in knockdown or knockout models if available.
• For immunohistochemistry (IHC-P), validate the antibody in tissues known to express MDH2, such as heart, brain, or skeletal muscle. Optimization of antigen retrieval and dilution may be needed.
• In ELISA applications, the antibody can be used for antigen detection; consider pairing with a compatible detection system and validating linearity and sensitivity for your specific assay.
• The antibody cross-reacts with mouse and rat MDH2, making it suitable for translational studies using these common model organisms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop procurement solution for IVD raw materials, including this validated anti-MDH2 rabbit monoclonal antibody, which supports research into mitochondrial metabolism and neurological disorders. Our extensive supply portfolio features optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets, helping diagnostic manufacturers and research laboratories accelerate assay development from concept to clinic.