Anti-MDH1 Polyclonal Antibody for WB and ELISA - P40925

Core Product Specifications and Parameters

Biological Background: MDH1 Function and Localization

• MDH1 is a cytoplasmic enzyme that catalyzes the reversible reduction of aromatic alpha-keto acids using NADH as a cofactor. Related references: PMID:2449162, PMID:3052244
• It plays essential roles in the malate-aspartate shuttle, transferring reducing equivalents across the mitochondrial membrane, and in the tricarboxylic acid cycle, supporting mitochondrial NADH supply for oxidative phosphorylation. Related references: PMID:31538237
• MDH1 can catalyze the reduction of 2-oxoglutarate to 2-hydroxyglutarate, a reaction that elevates intracellular reactive oxygen species (ROS) levels. Related references: PMID:34012073
• The protein localizes to the cytoplasm and cytosol, consistent with its metabolic functions.
• MDH1 is subject to multiple post-translational modifications including acetylation, methylation, phosphorylation, and ubiquitin conjugation, as indicated by proteomics-based PTM databases. Keywords relevant to its regulation include: 3D-structure, Alternative splicing, Disease variant, Epilepsy.
• Alternative splicing generates multiple isoforms, and mutations in MDH1 are associated with developmental and epileptic encephalopathy (DEE88).

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight of MDH1 is ~36 kDa; consider using appropriate gel percentage and transfer conditions. Validate detection in human, mouse, or rat lysates as the antibody shows cross-reactivity with these species.
• For ELISA, standard indirect or sandwich ELISA formats may be compatible; include appropriate controls and optimize coating antigen concentration and antibody dilution.
• The immunogen covers the full-length cytoplasmic MDH1; ensure that sample preparation preserves the native conformation for detection if conformational epitopes are important.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to high-quality IVD raw materials. For MDH1-related projects in metabolism and neurological disorders, our portfolio includes validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to accelerate assay development. Our one-stop service covers everything from target identification to clinical supply, ensuring you get the right materials at every stage.