Anti-MDH1 Monoclonal Antibody for WB, ELISA - P40925

Core Product Specifications and Parameters

Biological Background: MDH1 Function and Localization

• Malate dehydrogenase, cytoplasmic (MDH1) is an oxidoreductase that catalyzes the reversible oxidation of malate to oxaloacetate, utilizing NADH as a cofactor.
• MDH1 also acts as an aromatic alpha-keto acid reductase (KAR), reducing aromatic alpha-keto acids in the presence of NADH. Related references: PMID:2449162, PMID:3052244
• The enzyme plays a central role in the malate-aspartate shuttle and the tricarboxylic acid cycle, critical for supplying mitochondrial NADH for oxidative phosphorylation. Related references: PMID:31538237
• MDH1 catalyzes the reduction of 2-oxoglutarate to 2-hydroxyglutarate, a reaction associated with increased reactive oxygen species (ROS) production. Related references: PMID:34012073
• Predominantly localized in the cytoplasm and cytosol, MDH1 functions in key metabolic pathways.
• Alternative splicing yields multiple isoforms, and the protein is subject to post-translational modifications including acetylation, methylation, phosphorylation, and ubiquitination.
• Mutations in the MDH1 gene are linked to developmental and epileptic encephalopathy (DEE88) and other neurological diseases.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from the C-terminal region (amino acids 235-334) of human MDH1, which may confer high target specificity.
• For Western blot analysis, a specific band around 36 kDa is expected; optimize loading and detection conditions for your experimental system.
• In ELISA applications, the antibody may be used as a capture or detection reagent; validate pairing and sample matrix effects.
• Due to sequence homology, cross-reactivity with mouse and rat MDH1 is indicated; confirm performance across species of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing validated antibodies like this anti-MDH1 monoclonal antibody for metabolic and cancer research. We support diagnostic developers with bulk orders, custom antibody services, and ancillary reagents to accelerate assay development. Contact us to streamline your raw material procurement and technical needs.