Anti-MDC1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q14676

Core Product Specifications and Parameters

Biological Background: MDC1 Function and Localization

• MDC1 (Mediator of DNA damage checkpoint protein 1), also known as NFBD1 (Nuclear factor with BRCT domains 1), acts as a histone reader that specifically binds phosphorylated histone H2AX (γH2AX) at sites of DNA double-strand breaks, serving as a molecular scaffold for DNA repair and signal transduction proteins. Related references: PMID:12475977 PMID:12499369 PMID:12551934
• Upon DNA damage, MDC1 facilitates the recruitment and retention of DNA repair factors, including the MRN complex (via NBN) and TOPBP1, to damage foci; it is essential for the subsequent phosphorylation and activation of checkpoint kinases ATM, CHEK1, and CHEK2, leading to cell cycle arrest and stabilization of p53. Related references: PMID:12607004 PMID:12607005 PMID:15201865 PMID:16049003
• MDC1 contributes to chromosomal stability during mitosis by promoting TOPBP1 filament formation that tethers broken chromosomes, and it is required for homologous recombination repair by enabling NBN recruitment to DSBs. Related references: PMID:18411307 PMID:18582474 PMID:18583988 PMID:30898438
• The protein localizes primarily to the nucleus and associates with chromatin, particularly at damage-induced foci. It is highly expressed in testis, suggesting a role in meiotic or germ cell processes.
• MDC1 is a large phosphoprotein (~227 kDa) that undergoes multiple post-translational modifications, including acetylation, methylation, and ubiquitination, which regulate its interaction with DNA repair machinery.
• Alternative splicing generates multiple isoforms, and the protein contains tandem BRCT domains that mediate its binding to phosphorylated proteins at damage sites.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-350) of human MDC1; this region is outside the BRCT domains, and the antibody may recognize full-length and N-terminal fragments.
• For Western blot (WB), due to the large molecular weight (~227 kDa), consider using low-percentage SDS-PAGE gels (e.g., 6-8% separating gel) and sufficient transfer time.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), validate staining patterns in known positive tissues (e.g., testis) or DNA-damage-induced cell models.
• The antibody has been reported for ELISA, but optimal working dilutions must be determined empirically by the end user.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing validated antibodies, recombinant proteins, and ancillary reagents for diagnostic development. This anti-MDC1 polyclonal antibody supports research in DNA damage response and oncology, and we can assist with bulk orders, custom labeling, or sourcing related rare-target materials. From concept to clinic, CamelBio bridges your supply needs with expert technical support.