Anti-MCU Rabbit Polyclonal Antibody for WB and ELISA - Q8NE86

Core Product Specifications and Parameters

Biological Background: MCU Function and Localization

• MCU is the channel-forming and calcium-conducting subunit of the mitochondrial inner membrane calcium uniporter complex (uniplex), which mediates calcium uptake into the mitochondrial matrix. Related references: PMID:21685886, PMID:21685888, PMID:22822213
• It plays a central role in mitochondrial calcium homeostasis, regulating ATP production, cytoplasmic calcium signals, and activation of cell death pathways. Related references: PMID:21685886, PMID:21685888, PMID:22822213
• MCU channel activity is regulated by the calcium-sensor subunits MICU1 and MICU2 (or MICU3). Related references: PMID:24560927, PMID:26903221, PMID:30454562
• In cardiomyocytes, it buffers systolic calcium rises and acts as a key regulator of short-term mitochondrial calcium loading during acute stress, underlying a "fight-or-flight" response. Related references: PMID:25603276
• MCU contributes to mitochondrial permeability transition during ischemia-reperfusion injury (By similarity).
• It is involved in glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake. Related references: PMID:22829870, PMID:22904319
• Subcellular localization: Mitochondrion inner membrane, consistent with its role as a calcium channel.
• Keywords include Calcium channel, Calcium transport, Ion channel, Mitochondrion inner membrane, Phosphoprotein, and others, highlighting its functional and regulatory complexity.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody is a recombinant protein spanning amino acids 51–170 of human MCU (NP_612366.1); when establishing experimental conditions, consider using a blocking peptide or recombinant MCU protein to confirm band specificity in WB.
• For Western blot applications, validate the antibody’s reactivity in your cell or tissue lysate system (e.g., using mitochondrial fractions) and optimize primary antibody dilution; the predicted molecular weight is ~40 kDa.
• ELISA suitability has been specified by the supplier; for quantitative detection, consider paired antibody formats and validate against the target in relevant sample types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, researchers, and laboratories with one-stop access to IVD raw materials, technical services, and consultative support covering every stage from concept to clinic. For mitochondrial calcium signaling targets like MCU, CamelBio offers validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and custom sourcing of rare-target raw materials to streamline your assay development and research. Partner with us to leverage comprehensive procurement solutions and expert guidance for your IVD and molecular biology initiatives.