Anti-MCU Monoclonal Antibody (KO Validated) for WB, ELISA - Q8NE86

Core Product Specifications and Parameters

Biological Background: MCU Function and Localization

• MCU (Calcium uniporter protein, mitochondrial), also known as Coiled-coil domain-containing protein 109A, is the pore-forming and calcium-conducting subunit of the mitochondrial calcium uniporter complex (uniplex), essential for mitochondrial Ca²⁺ uptake (PubMed:21685886, PubMed:21685888, PubMed:22822213). Related references: PMID:21685886 PMID:21685888 PMID:22822213
• MCU channel activity is tightly regulated by the calcium-sensor subunits MICU1 and MICU2 (or MICU3) to prevent excessive calcium entry and maintain cellular homeostasis (PubMed:24560927, PubMed:26903221, PubMed:30454562). Related references: PMID:24560927 PMID:26903221 PMID:30454562
• Mitochondrial calcium homeostasis governed by MCU is critical for cellular energy metabolism, influencing ATP production, cytoplasmic calcium signaling, and the activation of cell death pathways (PubMed:21685886, PubMed:21685888, PubMed:22822213). Related references: PMID:21685886 PMID:21685888 PMID:22822213
• In cardiomyocytes, MCU buffers systolic calcium increases and mediates acute stress-induced ‘fight-or-flight’ mitochondrial calcium loading in pacemaker cells (PubMed:22822213, PubMed:25603276). Related references: PMID:22822213 PMID:25603276
• MCU participates in mitochondrial permeability transition during ischemia-reperfusion injury and contributes to synaptic vesicle endocytosis kinetics in neurons.
• MCU regulates glucose-dependent insulin secretion in pancreatic beta-cells and is involved in antigen processing and presentation (PubMed:22829870, PubMed:22904319). Related references: PMID:22829870 PMID:22904319
• Subcellular localization: Mitochondrion inner membrane, consistent with its role in direct mitochondrial calcium import.
• Post-translational modifications include acetylation, glutathionylation, and phosphorylation; the protein has a predicted molecular weight of approximately 40 kDa (UniProt: 39867 Da).

Experimental Guidance and Technical Tips

• For Western blot (WB), consider testing a range of primary antibody dilutions (e.g., 1:500–1:2000) to determine optimal signal-to-noise ratio in your sample system.
• The KO validated status indicates this antibody has been tested in knockout models; always include appropriate positive and negative controls to confirm specificity.
• Cross-reactivity with mouse and rat allows for use in rodent model studies; however, we recommend verifying performance in the relevant tissue or cell line.
• For ELISA applications, optimize coating concentration and detection conditions based on the specific assay format and antigen source.
• The immunogen corresponds to amino acids 51–170 of human MCU, encompassing a region likely involved in oligomerization; consider this when interpreting results in truncated or mutant constructs.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with convenient, one-stop access to validated IVD raw materials like this anti-MCU monoclonal antibody. Whether you need optimized antibody pairs, bulk ancillary reagents, or rare-target sourcing for mitochondrial biology and calcium signaling research, CamelBio supports your workflow from concept to clinic with reliable B2B procurement solutions.