Anti-MCT4/SLC16A3 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O15427

Core Product Specifications and Parameters

Biological Background: MCT4/SLC16A3 Function and Localization

• MCT4, encoded by the SLC16A3 gene (also designated MCT3), is a member of the solute carrier family 16 and functions as a proton-coupled monocarboxylate transporter.
• It mediates the transport of L-lactate and pyruvate across the plasma membrane, playing a critical role in metabolic homeostasis (PubMed:11101640, PubMed:23935841, PubMed:31719150). Related references: PMID:11101640, PMID:23935841, PMID:31719150
• Predominantly facilitates L-lactate efflux from highly glycolytic cells, contributing to pH regulation and metabolic cooperation (By similarity).
• Localized to the cell membrane, with basolateral distribution in polarized cells, enabling directional transport of substrates.
• Highly expressed in skeletal muscle, consistent with its role in lactate shuttling during exercise and metabolic stress.
• Post-translational modifications, including phosphorylation, may regulate transporter activity and membrane trafficking.
• Contains transmembrane helices characteristic of the major facilitator superfamily, underscoring its role as a transport protein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal intracellular region (aa 410–465) of human MCT4, offering potential high specificity; confirm specificity in your sample system.
• Predicted molecular weight is ~49 kDa; for western blot, consider using reducing conditions and account for possible band shifts due to post-translational modifications.
• For IHC-P, antigen retrieval and validation in positive control tissues (e.g., skeletal muscle) are recommended; optimize antibody dilution empirically.
• For ELISA applications, the antibody can be explored for target capture or detection; pairing with a matched detection antibody may enhance sensitivity.
• Cross-reactivity with mouse and rat is indicated; validate performance in each species and tissue type to ensure consistent results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, supporting diagnostic developers and researchers with validated antibodies, antigen pairs, and ancillary reagents. This MCT4/SLC16A3 polyclonal antibody addresses metabolic transporter studies and biomarker development, with bulk supply options available. For rare targets or tailored sourcing solutions, contact CamelBio to streamline your development pipeline.