Anti-MCT1/SLC16A1 Polyclonal Antibody for WB, IF/ICC, ELISA - P53985

Core Product Specifications and Parameters

Biological Background: MCT1/SLC16A1 Function and Localization

• Monocarboxylate transporter 1 (MCT1), encoded by the SLC16A1 gene, is a member of the solute carrier family 16 and is also known as MCT, HHF7, or MCT1D.
• Functions as a bidirectional proton-coupled transporter that catalyzes the rapid plasma membrane transport of monocarboxylates such as lactate, pyruvate, acetate, and ketone bodies (acetoacetate, beta-hydroxybutyrate), thereby contributing to intracellular pH maintenance. Related references: PMID:12946269, PMID:33333023
• Serves as a major lactate exporter and is implicated in cellular responses to a high-fat diet by modulating lactate and pyruvate levels, which influences central metabolic pathways, insulin secretion, and blood glucose homeostasis.
• Facilitates the protonated monocarboxylate form of succinate export during muscle cell acidification in exercising muscle and ischemic heart. Related references: PMID:32946811
• Adopts alternate outward- and inward-open conformation states; protonation and deprotonation of Asp309 is essential for this conformational change. Related references: PMID:33333023
• Localizes to the cell membrane, including basolateral and apical membranes, consistent with its role in directional transport across polarized epithelia.
• Widely expressed in human tissues, with protein detection in heart, blood lymphocytes, and monocytes.
• Classified under keywords such as phosphoprotein, alternative splicing, disease variant, and symport, indicating its regulatory complexity and clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 190-245 of human MCT1) is from a region that may exhibit high sequence conservation across human, mouse, and rat, consistent with the observed cross-reactivity; verify sequence homology if using other species.
• For western blotting, a specific band around 54 kDa is anticipated; however, validate detection using appropriate positive and negative controls, and be aware of potential isoforms or post-translational modifications that could shift mobility.
• In immunofluorescence/immunocytochemistry, expect plasma membrane staining; optimize fixation and permeabilization conditions to preserve the epitope and reduce background.
• When using this antibody in ELISA, consider testing a range of antibody dilutions and antigen coating concentrations to achieve optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a one-stop supply chain for IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies against metabolism-related targets such as MCT1/SLC16A1. Our portfolio covers bulk ancillary reagents, rare-target sourcing, and technical support to accelerate your project from concept to clinic. Contact us to explore customized procurement solutions for your next diagnostic development.