Anti-MCOLN2 (TRPML2) Rabbit Polyclonal Antibody for WB and ELISA - Q8IZK6

Core Product Specifications and Parameters

Biological Background: MCOLN2/TRPML2 Function and Localization

• MCOLN2 encodes mucolipin-2, also known as transient receptor potential channel mucolipin 2 (TRPML2), a member of the TRPML subfamily of ion channels.
• TRPML2 functions as a nonselective cation channel with inwardly rectifying activity, permeable to Ca2+ and Fe2+, and plays a role in the regulation of membrane trafficking events. Related references: PMID:19885840, PMID:19940139
• It may activate ARF6 and participate in the trafficking of GPI-anchored cargo proteins to the cell surface via the ARF6-regulated recycling pathway. Related references: PMID:17662026
• In the immune system, TRPML2 and TRPML1 may have redundant roles in specialized lysosomes of B cells in adaptive immunity, and in innate immunity, it may regulate chemokine secretion and macrophage migration (By similarity).
• TRPML2 can heteromerize with MCOLN1, potentially contributing to many lysosome-dependent cellular events in a tissue-specific manner. Related references: PMID:19885840
• Subcellular localization includes the cell membrane, late endosome membrane, lysosome membrane, and recycling endosome membrane, consistent with its roles in trafficking and ion homeostasis.
• Keywords from UniProt highlight its involvement in calcium transport, ion transport, protein transport, immunity, and lysosome biology.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 90-275 of human MCOLN2, located in the extracellular/luminal domain, potentially providing specificity for detection applications.
• For Western blot, the predicted molecular weight is 66 kDa; however, post-translational modifications may result in observed bands at different sizes. Include appropriate controls from human or rat samples.
• For ELISA, validate the antibody in the relevant sample matrix to confirm specificity and sensitivity.
• As a polyclonal antibody, it may recognize multiple epitopes and could be considered for applications requiring robust target detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with one-stop access to IVD raw materials, including this anti-MCOLN2/TRPML2 polyclonal antibody for ion channel and lysosomal research. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to streamline your development from concept to clinic. Our sourcing capabilities extend to rare targets, ensuring you find the right materials for your assays.