Anti-MCMBP Polyclonal Antibody for WB, ELISA - Q9BTE3

Core Product Specifications and Parameters

Biological Background: MCMBP Function and Localization

• MCMBP (Mini-chromosome maintenance complex-binding protein), also known as MCM-BP or C10orf119, is a regulator of DNA replication that associates with the MCM complex.
• During late S phase, MCMBP binds to the MCM complex and promotes its disassembly from chromatin, functioning as a key factor in unloading the pre-replication complex (pre-RC) from replicated DNA.
• It can dissociate the MCM complex in an ATP-independent manner, likely by destabilizing interactions among MCM subunits.
• MCMBP is required for sister chromatid cohesion, a critical process for proper chromosome segregation during cell division.
• The protein localizes to the nucleus, consistent with its role in DNA replication and chromatin dynamics.
• Keywords include alternative splicing, cell cycle, cell division, DNA replication, mitosis, and phosphoprotein, indicating regulation by phosphorylation and involvement in mitotic processes.
• The molecular weight is approximately 73 kDa (UniProt: 72,980 Da).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-240) of human MCMBP. This region may enable detection of full-length MCMBP. Validate the antibody's reactivity with your target sample to confirm specificity.
• For Western blot (WB), consider using nuclear extracts from human or mouse cells, given the nuclear localization of MCMBP, to enhance detection.
• In ELISA applications, recombinant MCMBP protein can be used as a positive control. Optimize antibody dilution and coating conditions for your specific assay setup.
• Cross-reactivity is reported with human and mouse; verify in other species if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted partner for IVD raw-material sourcing, offering validated antibody products like this anti-MCMBP polyclonal antibody for DNA replication and cell cycle research. We support diagnostic manufacturers and researchers with a comprehensive portfolio, including recombinant proteins, antibody pairs, and ancillary reagents, streamlining your path from concept to clinic. For rare targets or bulk requirements, our sourcing expertise ensures reliable access to high-quality raw materials.