Anti-MCM4 Monoclonal Antibody for WB, ELISA - P33991

Core Product Specifications and Parameters

Biological Background: MCM4 Function and Localization

• MCM4 is a core subunit of the MCM2-7 replicative helicase complex and the CDC45-MCM-GINS (CMG) helicase, driving DNA unwinding during replication. Related references: PMID:16899510, PMID:25661590, PMID:32453425
• The MCM2-7 ring’s ATPase activity is generated by composite active sites at subunit interfaces, where a conserved arginine finger motif is provided by the adjacent subunit. Related references: PMID:16899510, PMID:25661590, PMID:32453425
• MCM4 localizes to the nucleus and chromosomes, consistent with its role in genome duplication.
• Post-translational modifications including acetylation, phosphorylation, and ubiquitination may regulate MCM4 activity.
• MCM4 is also known as CDC21 homolog and P1-CDC21, reflecting its evolutionary conservation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (amino acids 764–863) of human MCM4; this region may facilitate recognition of full-length MCM4 across species.
• For Western blot, the expected band is around 97 kDa; consider using a resolving gel appropriate for high molecular weight proteins.
• In ELISA, titration against the immunizing peptide or recombinant MCM4 is recommended to optimize signal-to-noise ratio.
• Cross-reactivity is reported for human, mouse, and rat; performing sequence alignment for other species is advisable before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers with a comprehensive portfolio of validated antibodies, antigens, and ancillary reagents for DNA replication and cell cycle research. This anti-MCM4 monoclonal antibody is part of our expanding catalog of IVD raw materials, available along with bulk ordering and custom sourcing solutions. Contact us to streamline your assay development from concept to clinic.