Anti-MCM2 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P49736

Core Product Specifications and Parameters

Biological Background: MCM2 Function and Localization

• DNA replication licensing factor MCM2 is a core component of the MCM2-7 complex, which functions as the replicative helicase essential for DNA replication initiation and elongation in eukaryotic cells.
• Within the CDC45-MCM-GINS (CMG) helicase complex, MCM2 contributes to ATP-dependent DNA unwinding, with active ATPase sites formed through intersubunit interactions that drive helicase activity. Related references: PMID:32453425, PMID:34694004, PMID:34700328
• MCM2 is required for entry into S phase and for cell division, making it a critical regulator of cell cycle progression. Related references: PMID:8175912
• In terminally differentiated cochlear hair cells, MCM2 plays a role in development and can induce apoptosis. Related references: PMID:26196677
• Encoded by the MCM2 gene (aliases: BM28, CCNL1, CDCL1), this protein belongs to the MCM family of minichromosome maintenance proteins, conserved across eukaryotes.
• Subcellular localization is predominantly nuclear and associated with chromosomes during replication.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, which regulate its activity and stability. The protein contains ATP-binding, DNA-binding, and zinc-finger domains.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-288 of human MCM2 (NP_004517.2), covering the N-terminal region. This sequence is conserved across human, mouse, and rat, as indicated by the cross-reactivity data, making this antibody suitable for multi-species studies.
• For Western blot (WB), a major band at approximately 102 kDa is expected. Consider using nuclear extracts to enrich the target protein and validate the detection in your specific sample type.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), MCM2 exhibits nuclear staining. Optimize antigen retrieval and permeabilization steps based on your sample; validate in the relevant tissue or cell system.
• ELISA can be applied for quantitative detection of MCM2; consider pairing with a suitable capture or detection antibody for sandwich assay development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing solution for IVD raw materials, connecting diagnostic manufacturers and research labs with high-quality antibodies, including validated pairs and optimized monoclonals. For DNA replication and cell cycle research, we supply bulk reagents and can assist in rare target raw-material procurement. Partner with CamelBio to streamline your development pipeline from discovery to commercialization.