Anti-MCM10 Rabbit Polyclonal Antibody for WB and ELISA - Q7L590

Core Product Specifications and Parameters

Biological Background: MCM10 Function and Localization

• MCM10 is a conserved DNA replication initiation factor that coordinates the assembly of the MCM2-7 helicase with DNA polymerase alpha/primase at replication origins.
• It plays a critical role in preventing replication-associated DNA damage and maintaining genome integrity.
• MCM10 functions as a direct transcriptional target of ZBTB38 and is a key effector in RBBP6- and ZBTB38-mediated regulation of DNA replication and common fragile site stability. Related references: PMID:24726359
• The protein localizes to the nucleus, consistent with its role in DNA replication.
• MCM10 contains a zinc-finger domain and binds metal ions, important for its function and stability.
• Post-translational modifications include phosphorylation, ubiquitination, and isopeptide bond formation, which may regulate its activity.
• Alternative splicing of the MCM10 gene generates multiple isoforms, although their specific functions remain less characterized.
• The protein is also associated with disease variants, linking its dysfunction to human pathology.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region of human MCM10; antibody reactivity should be validated in the target species and experimental system.
• For Western blot, a band around 98 kDa is expected for the full-length MCM10 protein; consider using appropriate molecular weight markers.
• Given its polyclonal nature, this antibody may detect multiple isoforms or degradation products; antibody specificity should be confirmed with appropriate controls.
• For ELISA applications, the recombinant immunogen can be used as a positive control; optimal coating concentration and antibody dilution should be determined experimentally.
• Cross-reactivity with mouse MCM10 is indicated; validate performance in mouse samples if intended for non-human models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. This anti-MCM10 polyclonal antibody supports research in DNA replication and genome stability, and CamelBio can assist with sourcing complementary reagents and custom services for your development pipeline.