Anti-MCL1 Rabbit Polyclonal Antibody for WB - Q07820

Core Product Specifications and Parameters

Biological Background: MCL1 Function and Localization

• Encodes induced myeloid leukemia cell differentiation protein Mcl-1, also known as Bcl-2-like protein 3 (Bcl2-L-3) and Bcl-2-related protein EAT/mcl1.
• Functions as a key regulator of apoptosis and cell survival, maintaining viability without promoting proliferation; mediates effects through interactions with other apoptosis regulators.
• Alternative splicing yields isoforms with opposing activities: isoform 1 inhibits apoptosis, while isoform 2 promotes apoptosis.
• Subcellularly localized to the membrane, cytoplasm, mitochondrion, and nucleus (including nucleoplasm), reflecting its dynamic roles.
• Classified under multiple keywords including apoptosis, alternative splicing, phosphoprotein, transmembrane, and Ubl conjugation, highlighting its post-translational regulation and structural features.

Experimental Guidance and Technical Tips

• The immunogen was designed against a peptide within amino acids 100–200 of human MCL1 (NP_068779.1); consider that this region may be conserved across known isoforms, potentially enabling pan-isoform detection.
• For Western blot analysis, target the expected band around 37 kDa (the predicted molecular weight) and validate specificity in your appropriate human cell or tissue lysates.
• Include necessary positive and negative controls (e.g., lysates with known MCL1 expression status) to confirm specific reactivity and avoid misinterpretation from non‑specific bands.
• Optimize antibody dilution and incubation conditions for your specific sample system, as polyclonal antibodies may exhibit lot‑to‑lot variability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and research laboratories by providing a reliable, one‑stop sourcing channel for high‑quality IVD raw materials. This anti‑MCL1 polyclonal antibody, targeting a central apoptosis regulator, is representative of our commitment to supplying validated antibody pairs, optimized monoclonal and polyclonal formats, bulk ancillary reagents, and rare‑target raw materials. Whether you are building apoptosis‑related assays or scaling up production, we offer flexible procurement solutions to streamline your path from concept to clinic.