Anti-MCEE Rabbit Polyclonal Antibody for WB, ELISA - Q96PE7

Core Product Specifications and Parameters

Biological Background: MCEE Function and Localization

• MCEE (methylmalonyl-CoA epimerase, mitochondrial) is an isomerase enzyme involved in propionyl-CoA metabolism, catalyzing the epimerization of methylmalonyl-CoA.
• It is also known as DL-methylmalonyl-CoA racemase, reflecting its role in interconverting isomers.
• The protein localizes to the mitochondrion, as indicated by its transit peptide and subcellular localization data.
• MCEE is a metal-binding protein that requires cobalt for its enzymatic activity.
• Post-translational modifications include acetylation, as identified by proteomics studies.
• The protein has a molecular weight of approximately 19 kDa (calculated from sequence: 18,749 Da).
• It belongs to the isomerase family and serves as a reference proteome entry for Homo sapiens.
• The three-dimensional structure of MCEE has been determined, providing insights into its catalytic mechanism.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human MCEE (aa 1-176), suggesting the antibody may recognize both native and denatured forms; consider validating in the relevant sample system.
• For Western blotting, the predicted band is around 19 kDa; include appropriate molecular weight markers and validate with positive control lysates.
• Cross-reactivity with mouse is reported; verify performance in mouse samples if needed.
• For ELISA applications, optimize coating antigen and antibody dilution in your assay setup.
• As a polyclonal antibody, lot-to-lot consistency should be assessed for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting, covering every stage from concept to clinic. This anti-MCEE polyclonal antibody supports research in propionyl-CoA metabolism and mitochondrial disorders. Through our extensive supplier network, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare target raw materials to streamline your assay development and scale-up.