Anti-MCCC2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9HCC0

Core Product Specifications and Parameters

Biological Background: MCCC2 Function and Localization

• MCCC2 encodes the beta chain of 3-methylcrotonyl-CoA carboxylase (MCC), a mitochondrial biotin-dependent enzyme.
• The MCC holoenzyme is composed of alpha (biotin-containing) and beta (non-biotin-containing) subunits; MCCC2 provides the carboxyltransferase activity.
• MCC catalyzes the conversion of 3-methylcrotonyl-CoA to 3-methylglutaconyl-CoA, an essential step in the leucine degradation pathway and isovaleric acid catabolism.
• The protein localizes to the mitochondrial matrix, consistent with its role in branched-chain amino acid metabolism.
• Post-translational modifications include acetylation, and multiple isoforms are produced by alternative splicing.
• Mutations in MCCC2 are associated with 3-methylcrotonyl-CoA carboxylase deficiency, a disorder of leucine metabolism.
• The protein contains ATP-binding and nucleotide-binding domains, essential for its ligase activity.
• The full-length precursor has a predicted mass of ~61 kDa, matching the observed 61 kDa band in western blotting.

Experimental Guidance and Technical Tips

• For western blotting, expect a specific band at approximately 61 kDa; using reducing conditions is recommended.
• For immunohistochemistry (IHC-P), consider heat-induced antigen retrieval and testing various primary antibody dilutions.
• For immunofluorescence (IF/ICC), mitochondrial staining patterns should be observed; co-stain with a mitochondrial marker for confirmation.
• For ELISA, use the antibody as a capture or detection reagent after appropriate titration.
• Validate the antibody in your specific sample system and consider including positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. This anti-MCCC2 polyclonal antibody supports research into mitochondrial metabolism and genetic disorders of leucine catabolism. Beyond this antibody, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development and production workflows.