Anti-MCAT Rabbit Polyclonal Antibody for WB and ELISA - Q8IVS2

Core Product Specifications and Parameters

Biological Background: MCAT Function and Localization

• MCAT (malonyl-CoA-acyl carrier protein transacylase) is a mitochondrial enzyme that catalyzes the transfer of a malonyl group from malonyl-CoA to the phosphopantetheine arm of the mitochondrial acyl carrier protein (ACP, NDUFAB1), suggesting a role in mitochondrial fatty acid biosynthesis.
  Related references: PMID:12882974 PMID:19549604

• Additionally, MCAT functions as an assembly factor for the mitochondrial small ribosomal subunit (mt-SSU), highlighting its dual role in lipid metabolism and mitochondrial protein synthesis.
  Related references: PMID:36482135

• The protein contains a transit peptide and localizes to the mitochondrion.

• MCAT is classified as a transferase involved in fatty acid biosynthesis and lipid metabolism.

• Alternative splicing of the MCAT gene may produce multiple transcript variants, though functional differences remain to be fully characterized.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein corresponding to amino acids 191-390 of human MCAT. Validate specificity against full-length protein and relevant isoforms in your sample system.
• Recommended applications include Western blot (WB) and ELISA. For WB, the predicted molecular weight is ~43 kDa; adjust gel percentage and transfer conditions accordingly.
• Cross-reactivity is reported for human; for experiments with other species, confirm sequence homology to avoid off-target binding.
• As a rabbit polyclonal antibody, performance may vary between lots; include appropriate controls when using a new batch.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD manufacturers and research laboratories, offering streamlined access to essential raw materials such as this anti-MCAT rabbit polyclonal antibody. Beyond antibodies, we provide validated antibody pairs, bulk ancillary reagents, and custom procurement services for rare or specialized targets, supporting your development workflow from concept to clinic.