Anti-MC5 Receptor Rabbit Monoclonal Antibody for WB, ELISA - P33032

Core Product Specifications and Parameters

Biological Background: MC5 Receptor Function and Localization

• MC5R is a G protein-coupled receptor that binds melanocyte-stimulating hormones (α-, β-, γ-MSH) and corticotropin (ACTH), all derived from the POMC precursor. Related references: PMID:37524700 PMID:8396929
• Upon activation, MC5R couples to the G(s) protein, stimulating adenylate cyclase and increasing intracellular cAMP levels. Related references: PMID:37524700
• It also signals through a PI3K-dependent ERK1/2 pathway, independent of cAMP. Related references: PMID:19428994
• The natural melanocortin potency order at MC5R is α-MSH > ACTH > β-MSH > γ-MSH. Related references: PMID:8396929
• MC5R plays a role in immune response, temperature regulation, and exocrine gland function.
• The receptor localizes to the cell membrane and contains structural features typical of GPCRs: seven transmembrane domains, disulfide bonds, palmitoylation, and glycosylation.
• Expression is detected in the brain, but not in melanoma cells.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1-100). Confirm specificity by pre-adsorbing with the immunizing peptide or testing in MC5R knockout models.
• For WB, the predicted molecular weight is 37 kDa; optimize loading and antibody dilution using positive control lysates from brain tissue or MC5R-transfected cells.
• This antibody has been applied in ELISA; consider pairing with a detection antibody for a sandwich ELISA format.
• Cross-reactivity with mouse and rat is reported, enabling translational studies across species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies for melanocortin receptor targets like MC5R. Our one-stop supply chain covers antibody pairs, ancillary reagents, and custom bulk sourcing to accelerate your diagnostic development from concept to clinic.