Anti-MC2R Polyclonal Antibody for WB and ELISA - Q01718

Core Product Specifications and Parameters

Biological Background: MC2R Function and Localization

• MC2R encodes the adrenocorticotropic hormone receptor (ACTHR), also known as melanocortin receptor 2 (MC2-R), a member of the melanocortin receptor family.
• It is a G protein-coupled receptor (GPCR) for corticotropin/ACTH, primarily expressed in the adrenal cortex. Upon ACTH binding, it couples to G(s) protein, activates adenylate cyclase, and stimulates the cAMP-dependent signaling pathway, MAPK cascade, and PKA pathway, leading to steroidogenic factor 1 (NR5A1)-mediated transcriptional activation. Related references: PMID:1325670, PMID:17596328, PMID:36588120
• Activation of MC2R facilitates the release of adrenal glucocorticoids, including cortisol and corticosterone. Related references: PMID:12213892, PMID:8636348
• MC2R is localized to the cell membrane, consistent with its role as a transmembrane receptor.
• It is required for fetal and neonatal adrenal gland development (By similarity).
• Tissue specificity includes expression in melanocytes and corticoadrenal tissue.
• Structurally, MC2R contains disulfide bonds, is glycosylated and palmitoylated, undergoes Ubl conjugation, and binds calcium. It is associated with disease variants (UniProt keywords).
• The molecular weight of MC2R is approximately 34 kDa (UniProt calculated mass 33927 Da), aligning with the observed protein weight.

Experimental Guidance and Technical Tips

• The antibody was generated using an immunogen corresponding to the N-terminal extracellular domain (amino acids 1-100) of human MC2R. This region may be accessible for detection in native conditions.
• For Western blot (WB), a band around 34 kDa is anticipated. Validate the antibody in relevant sample types such as human adrenal cortex lysates or mouse adrenal tissue, considering the reported cross-reactivity.
• For ELISA applications, consider employing the antibody as a capture or detection reagent against MC2R peptide or recombinant protein; optimal working concentrations should be determined empirically.
• The reported reactivity includes human and mouse; cross-reactivity with other species should be independently verified if needed.
• Suitability for other applications like immunohistochemistry (IHC) or immunofluorescence (IF) has not been established; end users should perform appropriate validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-MC2R antibody supports research into adrenocortical function, glucocorticoid regulation, and GPCR signaling. Contact us for customized sourcing and technical support to accelerate your assay development.