Anti-MC1 Receptor Monoclonal Antibody for WB, ELISA - Q01726

Core Product Specifications and Parameters

Biological Background: MC1 Receptor Function and Localization

• MC1R is a G protein-coupled receptor that binds melanocyte-stimulating hormones (alpha, beta, gamma-MSH) and adrenocorticotropic hormone/ACTH, which are peptide products of the POMC precursor protein. Related references: PMID:11442765, PMID:11707265, PMID:1325670
• Upon activation, MC1R couples with the G(s) protein, stimulating adenylate cyclase and activating the cAMP-dependent signaling pathway. Related references: PMID:11707265, PMID:1325670, PMID:16463023
• This activation promotes melanogenesis, resulting in the production of eumelanin (black/brown) and pheomelanin (red/yellow) in melanocytes. Related references: PMID:11707265, PMID:1325670, PMID:16463023
• MC1R interacts with G protein-coupled receptor opsin 3/OPN3, which couples to G(i) proteins and inhibits the alpha-MSH-induced cAMP response, thereby reducing melanin synthesis. Related references: PMID:31097585
• Binding to Agouti/ASP precludes alpha-MSH-induced signaling, thereby downregulating melanogenesis.
• Interaction with MGRN1 displaces the G(s) protein, further suppressing MC1R signaling. Related references: PMID:19737927
• MC1R is localized at the cell membrane.
• It is expressed in melanocytes and corticoadrenal tissue. Related references: PMID:1325670, PMID:31097585

Experimental Guidance and Technical Tips

• The immunogen targeting the N-terminal extracellular domain (amino acids 1-100) may be suitable for detecting MC1R under denaturing (WB) and native (ELISA) conditions, given the receptor's transmembrane topology.
• For WB experiments, consider using membrane protein extraction protocols and validate in melanocyte or corticoadrenal cell lines that endogenously express MC1R.
• The reported cross-reactivity with mouse and rat suggests conserved epitopes; validate in the species of interest with appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic developers and research teams streamlined access to validated IVD raw materials across the entire development pipeline. For melanocortin receptor research or pigmentation-related assay development, we can supply optimized monoclonal antibodies, matched antibody pairs, and bulk ancillary reagents. Whatever your MC1R sourcing needs—from rare target procurement to custom technical support—CamelBio bridges the gap from concept to clinic.