Anti-MBTPS1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q14703

Core Product Specifications and Parameters

Biological Background: MBTPS1 Function and Localization

• Membrane-bound transcription factor site-1 protease (MBTPS1), also known as S1P, PCSK8, and SKI-1, is a serine protease of the subtilisin/kexin family that plays a critical role in regulated intramembrane proteolysis.
• Catalyzes the first cleavage step required for the activation of sterol regulatory element-binding proteins (SREBPs), cleaving after the sequence Arg-Ser-Val-Leu to regulate cholesterol and fatty acid synthesis (PubMed:12782636).
  Related references: PMID:12782636
• Processes a diverse set of substrates, including ATF6, BDNF, GNPTAB, FAM20C, and CREB3L2, thereby contributing to ER stress responses, lysosomal enzyme trafficking, osteoblast differentiation, and collagen secretion (PubMed:10644685, PubMed:21719679, PubMed:34349020, PubMed:30046013).
  Related references: PMID:10644685 PMID:21719679 PMID:34349020 PMID:30046013
• Resides predominantly on the endoplasmic reticulum and Golgi apparatus membranes, with reported localization to mitochondria and as a secreted form, consistent with its multifaceted roles.
• Undergoes autocatalytic processing from its zymogen precursor and features glycosylation and phosphorylation; annotated as a glycoprotein, phosphoprotein, and zymogen.
• Widely expressed in human tissues, supporting its broad regulatory importance in various cell types.
• Genetic mutations in MBTPS1 are linked to disorders such as dwarfism, cataract, and hypotrichosis, highlighting its essential function in development.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (aa 953-1052) of human MBTPS1; the antibody is expected to recognize the full-length protein and any cleavage fragments retaining this epitope.
• For Western blot, a band near 118 kDa is anticipated, but additional bands may appear due to post-translational modifications or proteolytic processing. Consider running reducing SDS-PAGE and optimizing blocking conditions.
• In immunohistochemistry on paraffin-embedded tissues, antigen retrieval (e.g., citrate buffer, pH 6) is commonly employed; validate in the relevant tissue system as staining intensity can vary.
• Cross-reactivity with mouse and rat MBTPS1 is predicted based on immunogen sequence homology; confirm performance in animal model experiments.
• For ELISA, the antibody can be used as a capture or detection reagent; appropriate pair testing and assay optimization are advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with comprehensive IVD raw material sourcing, including validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare target procurement for protease and lipid metabolism research. Our supply capabilities facilitate the development of assays targeting MBTPS1-related pathways, ensuring lot-to-lot consistency. As a one-stop sourcing bridge, we accelerate your project from concept to clinic with integrated access to critical materials and technical expertise.