Anti-MBTPS1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q14703

Core Product Specifications and Parameters

Biological Background: MBTPS1 Function and Localization

• MBTPS1 encodes a membrane-bound serine protease that cleaves substrates after hydrophobic or small residues with Arg/Lys at P4. Key substrates include SREBF1/2, ATF6, BDNF, GNPTAB, and FAM20C, and it catalyzes the first step in activating SREBPs and ATF6, linking it to cholesterol/lipid metabolism and the unfolded protein response. Related references: PMID:10644685, PMID:12782636, PMID:21719679

• The protease mediates cleavage of GNPTAB into alpha/beta subunits crucial for lysosomal biogenesis, and processes FAM20C to promote its secretion and kinase activity for osteoblast differentiation. Related references: PMID:21719679, PMID:34349020

• It regulates M6P-dependent Golgi-to-lysosome trafficking and mega vesicle-mediated collagen transport via activation of CREB3L2/BBF2H7. Related references: PMID:30046013

• MBTPS1 promotes FAD binding to the ETFA/ETFB complex, influencing mitochondrial respiratory chain function. Related references: PMID:35362222

• Upon DNA damage or ER stress, MBTPS1 mediates proteolytic activation of CREB3L1, leading to cell-cycle arrest. Related references: PMID:16417584

• The protein localizes to the endoplasmic reticulum and Golgi membranes, can be secreted, and is also found in mitochondria. It is widely expressed across tissues.

• Belonging to the subtilisin/kexin family, MBTPS1 undergoes autocatalytic cleavage for zymogen activation, requires calcium, and is subject to glycosylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 803-1052 of human MBTPS1, a region that may target specific domains; consider sequence alignment to confirm suitability for your species of interest.
• For Western blot (WB), the predicted molecular weight is ~118 kDa; optimize gel percentage and transfer conditions for high-molecular-weight proteins.
• As a polyclonal antibody, batch-to-batch consistency may vary; validate reactivity and specificity in your relevant sample system.
• Cross-reactivity with mouse is reported, enabling use in mouse models; however, verify expression levels in your model.
• For immunocytochemistry (IF/ICC), note that MBTPS1 localizes to ER/Golgi; co-stain with appropriate organelle markers for confirmation.
• In ELISA applications, titrate the antibody to determine optimal working concentration for your assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw-material sourcing partner, supplying high-quality antibodies and reagents for critical targets such as MBTPS1, a protease pivotal in cholesterol metabolism, ER stress, and lysosomal function. Our comprehensive portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and expert support for rare target sourcing. Accelerate your IVD assay development with CamelBio's end-to-end procurement solutions.