Anti-MBL2 Polyclonal Antibody for WB, IHC-P, ELISA - P11226

Core Product Specifications and Parameters

Biological Background: MBL2 Function and Localization

• MBL2, also known as mannose-binding protein C or collectin-1, is a calcium-dependent lectin that acts as a pattern recognition receptor, specifically binding mannose moieties on pathogen surfaces to initiate the lectin pathway of complement activation. Related references: PMID:22966085, PMID:2573758, PMID:6643429
• Upon ligand binding, MBL2 activates MASP1 serine protease, triggering a proteolytic cascade that leads to increased phagocytosis and signaling that strengthens the adaptive immune response. Related references: PMID:14515269, PMID:7634089
• MBL2 has been shown to bind DNA, suggesting a role in clearing apoptotic cells and modulating inflammatory responses. Related references: PMID:15145932
• During SARS-CoV-2 infection, MBL2 activates the complement lectin pathway, leading to inhibition of viral infection and reduction of the induced inflammatory response. Related references: PMID:35102342
• The protein is secreted and can also be found on the cell surface; it is primarily produced in the liver and circulates in plasma.
• Structurally, MBL2 contains a signal peptide, a collagen-like domain with coiled-coil regions, and a C-terminal carbohydrate recognition domain; it forms oligomeric complexes stabilized by disulfide bonds.
• Post-translational modifications include hydroxylation of proline and lysine residues within the collagen-like domain, essential for structural stability.
• Keywords associated with MBL2 highlight its roles in innate immunity, complement activation lectin pathway, calcium and metal binding, and its presence in the secretome.

Experimental Guidance and Technical Tips

• The immunogen used for this polyclonal antibody corresponds to amino acids 21-248 of human MBL2, covering the extracellular region, which may allow detection of both secreted and cell-associated forms.
• For Western blot (WB) analysis, the predicted molecular weight is 26 kDa; consider using human liver or plasma as positive controls, and validate the antibody in the relevant sample system.
• In immunohistochemistry (IHC-P), antigen retrieval conditions may need optimization; inclusion of appropriate positive and negative tissue controls is recommended.
• For ELISA, the antibody can be used as a capture or detection reagent, but pairing and titration must be empirically determined.
• Cross-reactivity with mouse MBL2 has been reported, but reactivity with other species should be verified if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-MBL2 polyclonal antibody supports research into the complement system and innate immunity, complementing our portfolio of lectin-pathway-related products. For specialized needs, including rare target raw-material sourcing and technical consultation from concept to clinic, our team is ready to assist.