Anti-MBD3 Rabbit Polyclonal Antibody for WB, ELISA - O95983

Core Product Specifications and Parameters

Biological Background: MBD3 Function and Localization

• MBD3 is a component of the NuRD (nucleosome remodeling and deacetylase) complex, which remodels chromatin and regulates transcription by recruiting histone deacetylases and DNA methyltransferases.
  Related references:
  PMID:12124384 PMID:16428440 PMID:28977666

• It acts as a transcriptional repressor and plays a role in gene silencing, likely as part of the NuRD complex.
  Related references:
  PMID:10947852 PMID:18644863

• MBD3 itself does not bind methylated CpG dinucleotides, but it can bind unmethylated DNA with low affinity, distinguishing it from other methyl-CpG-binding domain proteins.
  Related references:
  PMID:12124384 PMID:16428440 PMID:24307175

• Localized to the nucleus and associated with chromosomes, MBD3 is essential for NuRD complex stability and chromatin targeting.

• Post-translational modifications include phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation, which may regulate its activity or interactions.

• The MBD3 gene undergoes alternative splicing, producing multiple isoforms that may have distinct functions or expression patterns.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1–255), which includes the methyl-CpG-binding domain and parts of the coiled-coil region; this design may help detect multiple isoforms.
• For Western blot (WB), a band at approximately 33 kDa is expected; validate the detected band in your sample system and consider loading controls.
• For ELISA, this polyclonal antibody can be used as a capture or detection reagent; titrate to determine optimal working concentrations.
• Since cross-reactivity with mouse is reported, the antibody may be suitable for mouse model studies; however, validate in the relevant species and tissue context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a seamless, one-stop sourcing bridge for diagnostic manufacturers and research institutes, offering validated IVD raw materials from leading suppliers like Abclonal. This anti-MBD3 rabbit polyclonal antibody supports epigenetics and chromatin remodeling research, backed by CamelBio's expertise in supplying high-quality antibodies, bulk ancillary reagents, and technical consultation. Whether you need rare-target antibodies, custom monoclonal development, or scalable production reagents, CamelBio connects your project from concept to clinic with reliable supply chain solutions.