Anti-MBD3 Monoclonal Antibody for WB, IHC-P, ELISA - O95983

Core Product Specifications and Parameters

Biological Background: MBD3 Function and Localization

• MBD3 (Methyl-CpG-binding domain protein 3) is a key component of the NuRD (nucleosome remodeling and deacetylase) complex, which mediates chromatin remodeling and transcriptional repression. Related references: PMID:12124384, PMID:16428440, PMID:28977666

• Unlike other methyl-CpG-binding domain proteins, MBD3 does not bind methylated DNA directly but can interact with unmethylated CpG dinucleotides, contributing to its functional specificity. Related references: PMID:24307175

• MBD3 functions as a transcriptional repressor, playing a critical role in gene silencing through recruitment of histone deacetylases and DNA methyltransferases. Related references: PMID:10947852, PMID:18644863

• The protein is localized to the nucleus and associated with chromosomes, consistent with its role in chromatin dynamics.

• MBD3 undergoes post-translational modifications including phosphorylation and ubiquitination, which may regulate its activity and interactions.

• Alternative splicing generates multiple isoforms of MBD3, potentially diversifying its functional roles in different cellular contexts.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1-100), suggesting a linear epitope suitable for denaturing Western blot and possibly IHC-P after appropriate antigen retrieval.
• For WB, consider optimizing antibody concentration in the range of 1:500–1:2000 using a positive control lysate (e.g., HeLa, HEK293) and validate with knockout or siRNA-treated samples.
• In IHC-P, epitope retrieval with citrate buffer (pH 6.0) or EDTA (pH 8.0) may be beneficial; perform a pilot experiment with known positive and negative tissues.
• For ELISA, pairing with a suitable detection antibody and using a blocking buffer compatible with the assay format is recommended; test several coating antigen concentrations.
• Cross-reactivity with mouse and rat has been reported; however, individual lot validation in the target species is advised for critical applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop sourcing partner for IVD raw materials, offering validated antibodies, optimized antibody pairs, and bulk ancillary reagents. Our MBD3 Rabbit mAb supports epigenetic and chromatin research, and we can assist with sourcing rare targets and custom production needs. Let CamelBio bridge the gap from concept to clinic with reliable raw material supply and technical expertise.