Anti-MBD2 Rabbit Monoclonal Antibody for WB, ELISA - Q9UBB5

Core Product Specifications and Parameters

Biological Background: MBD2 Function and Localization

• MBD2 specifically binds methylated CpG islands in DNA, serving as a key reader of DNA methylation marks.
  Related references:
  PMID:9774669

• It binds hemimethylated DNA and recruits histone deacetylases and DNA methyltransferases, thereby reinforcing chromatin compaction and transcriptional silencing.
  Related references:
  PMID:10947852, PMID:24307175, PMID:10471499

• As a core component of the NuRD (nucleosome remodeling and deacetylase) complex, MBD2 contributes to chromatin remodeling and robust gene repression.
  Related references:
  PMID:16428440, PMID:28977666

• It functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression.
  Related references:
  PMID:16415179

• MBD2 may enhance activation of some unmethylated cAMP-responsive promoters, indicating context-dependent regulatory functions.
  Related references:
  PMID:12665568

• The protein localizes predominantly to the nucleus and chromosomes, consistent with its role in transcriptional regulation.

• It is highly expressed in brain, heart, kidney, stomach, testis, and placenta.

• MBD2 undergoes alternative splicing and post-translational modifications, including phosphorylation, which may modulate its activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100–200 of human MBD2, a region likely within the methyl-CpG binding domain; therefore, the antibody may recognize conserved epitopes in mouse MBD2, as indicated by cross-reactivity data.
• For Western blotting, anticipate a band at approximately 43 kDa in human and mouse cell lysates. Validate with positive controls such as testis or brain tissue.
• In ELISA, consider using purified recombinant MBD2 or nuclear extracts from cells with confirmed MBD2 expression. Titrate antibody concentration to optimize signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio accelerates diagnostic development by providing comprehensive access to IVD raw materials, from validated antibody pairs to optimized monoclonal/polyclonal antibodies and bulk ancillary reagents. This anti-MBD2 antibody supports epigenetics and oncology research, enabling studies into methylation biomarkers and chromatin remodeling. Whether you need rare-target sourcing or end-to-end technical consultation, CamelBio bridges the gap between concept and clinic.