Anti-Matrix Metalloproteinase-11 (MMP11/Stromelysin-3) Rabbit Monoclonal Antibody for WB, ELISA - P24347

Core Product Specifications and Parameters

Biological Background: MMP11 Function and Localization

• MMP11, also known as Stromelysin-3 or Matrix metalloproteinase-11 (MMP-11), belongs to the matrix metalloproteinase family of zinc-dependent endopeptidases involved in extracellular matrix remodeling.
• Primarily implicated in the progression of epithelial malignancies, such as breast carcinomas, by facilitating tumor invasion and metastasis.
• Encoded by the MMP11 gene (GeneID: 4320) in humans, it is expressed as a secreted zymogen that requires proteolytic cleavage for activation.
• Localizes to the extracellular space and extracellular matrix after secretion, where it interacts with substrates to modulate the tumor microenvironment.
• Specifically expressed in stromal cells surrounding breast carcinomas, rather than in the tumor cells themselves, indicating a paracrine role in cancer.
• Contains characteristic structural domains: a signal peptide, a pro-domain, a catalytic domain with a zinc-binding site, and a hemopexin-like repeat domain; requires calcium and zinc ions for stability and activity.
• Post-translational modifications include disulfide bond formation and cleavage on a pair of basic residues to generate the active enzyme.

Experimental Guidance and Technical Tips

• The immunogen was designed using a synthetic peptide corresponding to the C-terminal region (amino acids 389-488) of human MMP11, which lies outside the catalytic domain; this may improve detection of both precursor and mature forms.
• For Western blotting, a band at approximately 55 kDa is expected under reducing conditions; consider optimizing gel percentage and transfer conditions for this molecular weight.
• This antibody exhibits cross-reactivity with mouse and rat MMP11, making it suitable for studies in rodent models; however, it is recommended to validate reactivity in your specific experimental system.
• ELISA application can be explored using recombinant MMP11 or cell culture supernatants, but optimal coating and detection antibody concentrations should be empirically determined.
• Since MMP11 is secreted, supernatants or extracellular matrix extracts may provide richer detection than whole-cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support diagnostic development. This anti-MMP11 rabbit monoclonal antibody is sourced from capillary electrophoresis technology, suitable for cancer biomarker research applications. For rare target raw-material sourcing and technical consultation, CamelBio serves as a one-stop partner from concept to clinic.