Anti-Matrin 3 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P43243

Core Product Specifications and Parameters

Biological Background: Matrin-3 Function and Localization

• Matrin-3 is a nuclear matrix protein encoded by the MATR3 gene (also known as KIAA0723), and is implicated in transcriptional regulation and nuclear matrix organization.
• It associates with the SFPQ-NONO heteromer to mediate nuclear retention of defective RNAs.
• As a component of the HDP-RNP complex, Matrin-3 facilitates IRF3 phosphorylation and activates innate immune signaling via the cGAS-STING pathway.
  Related references:
  PMID:28712728
• Matrin-3 binds to N6-methyladenosine (m6A)-modified mRNAs and enhances MYC mRNA stability, thereby sustaining MYC protein levels.
  Related references:
  PMID:32245947
• It may bind to specific miRNA hairpins, potentially influencing miRNA processing or function.
  Related references:
  PMID:28431233
• The protein undergoes multiple post-translational modifications including phosphorylation, acetylation, and ubiquitination (Ubl conjugation), and contains zinc-finger domains, RNA-binding motifs, and repeat regions.
• Mutations in MATR3 are linked to amyotrophic lateral sclerosis (ALS) and other neurodegenerative disorders, highlighting its role in neuronal health.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 747–847 of human Matrin-3; this region is conserved in mouse and rat, supporting the reported cross-reactivity. Validate cross-reactivity in your specific sample types.
• For Western blot, expect a band at approximately 95 kDa; optimize antibody dilution using a titration series with your sample system.
• In IHC-P, consider nuclear antigen retrieval protocols; test with known positive tissues (e.g., HeLa or brain samples).
• For IF/ICC, confirm nuclear matrix localization; fixation and permeabilization conditions should be optimized.
• ELISA may be used for quantitative detection; include appropriate controls and standard curves.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, bridging the gap from concept to clinic. For researchers focused on nuclear architecture, RNA biology, or neurodegeneration, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. Whether you need high-quality Matrin-3 antibodies for diagnostic development or complementary nucleoprotein and immune-signaling reagents, CamelBio streamlines your procurement with reliable, one-stop support.