Anti-Matrin 3 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P43243

Core Product Specifications and Parameters

Biological Background: Matrin-3 Function and Localization

• Matrin-3 is a nuclear matrix protein implicated in transcription and internal fibrogranular network formation.
• In complex with SFPQ-NONO, it promotes nuclear retention of defective RNAs.
• Assembles into the HDP-RNP complex to activate IRF3 via cGAS-STING, contributing to innate antiviral immunity. Related references: PMID:28712728
• Binds N6-methyladenosine (m6A)-modified mRNAs, stabilizing MYC transcripts. Related references: PMID:32245947
• May bind specific miRNA hairpins, potentially influencing miRNA metabolism. Related references: PMID:28431233
• Predominantly localizes to the nucleus matrix and contains zinc-finger and RNA recognition motifs.
• Undergoes diverse post-translational modifications such as acetylation, phosphorylation, and ubiquitination.
• Genetic mutations are associated with amyotrophic lateral sclerosis 21 (ALS21) and vocal cord and pharyngeal weakness with distal myopathy (VCPDM).

Experimental Guidance and Technical Tips

• Immunogen design: The antibody was raised against a synthetic peptide spanning residues 700–800 of human Matrin-3; expect detection of full-length Matrin-3 at ~95 kDa.
• Western blot (WB): Perform standard SDS-PAGE and transfer protocols; include relevant positive and negative controls to confirm specificity.
• Immunohistochemistry (IHC-P) and Immunofluorescence (IF/ICC): Nuclear matrix staining is anticipated; validate staining patterns in tissue or cell lines of interest.
• ELISA: Suitable for direct or indirect ELISA setups; consider optimizing coating antigen concentrations and detection antibody working dilutions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, supporting diagnostic developers from concept to clinic. For Matrin-3-focused research in neurodegeneration and RNA biology, we supply validated antibody pairs, recombinant antibodies, and ancillary reagents in bulk. Our sourcing network ensures access to rare targets and scalable, traceable raw materials for assay development.