Anti-Matrilin-2 (MATN2) Rabbit Polyclonal Antibody for WB and ELISA - O00339

Core Product Specifications and Parameters

Biological Background: Matrilin-2 Function and Localization

• Matrilin-2 is an extracellular matrix protein encoded by the MATN2 gene (Gene ID: 4147) and belongs to the matrilin family of non-collagenous adapter proteins.
• It is secreted into the extracellular space and functions primarily in matrix assembly, contributing to the structural organization of connective tissues.
• The protein features a modular architecture with von Willebrand factor A-like domains, EGF-like repeats, and a coiled-coil region that mediates oligomerization.
• Alternative splicing generates multiple isoforms, which may exhibit distinct expression patterns or functional roles.
• Post-translational modifications include glycosylation and disulfide bond formation, essential for proper folding and stability.
• The protein contains a signal peptide for secretion and is characterized by repeated domain structures important for intermolecular interactions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic C-terminal peptide (aa 800–end) of human MATN2 isoform NP_001304677.1; therefore, the antibody may detect isoforms that include this region. Verify specificity if multiple isoforms are expressed in your system.
• For Western blot, expect a band at approximately 107 kDa under standard reducing and denaturing conditions. Optimize sample preparation for your tissue or cell type, as isoform variance may lead to additional bands.
• In ELISA, this antibody can be used as a detection or capture reagent. Perform appropriate cross-validation with recombinant antigen or established controls to ensure assay performance.
• As a polyclonal antibody, lot-to-lot consistency should be confirmed for quantitative applications. Consider affinity purification or cross-adsorption if non-specific binding is observed.
• Cross-reactivity with human and rat has been documented. For other species, align the immunogen peptide sequence to predict reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes streamlined access to IVD raw materials, including validated antibodies like this anti-MATN2 polyclonal antibody for extracellular matrix studies. Whether you require bulk supply, monoclonal alternatives, or ancillary reagents for matrix assembly research, our one-stop platform supports your entire development pipeline from concept to clinic.