Anti-MATN4 Rabbit Polyclonal Antibody for WB and ELISA - O95460

Core Product Specifications and Parameters

Biological Background: MATN4 Function and Localization

• MATN4 encodes matrilin-4, a member of the matrilin family of non-collagenous extracellular matrix proteins.
• It is a major component of the extracellular matrix of cartilage, contributing to matrix organization and biomechanical properties.
• The protein is secreted, with a signal peptide, multiple EGF-like domains, and a coiled-coil region that facilitates multimerization.
• Alternative splicing produces several isoforms, adding functional diversity.
• Tissue expression is observed in embryonic kidney, lung, and placenta, suggesting developmental roles beyond cartilage.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation, critical for protein stability and function.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 221–540, a region containing potential EGF-like domains that are conserved across human, mouse, and rat, consistent with the observed cross-reactivity.
• In Western blotting, the predicted molecular weight is ~68 kDa, but actual migration may differ due to glycosylation; use reducing conditions for optimal results.
• For ELISA applications, this polyclonal antibody can serve as either capture or detection reagent; titrations and buffer optimization are recommended.
• Validate antibody performance in relevant models, such as cartilage tissue extracts or chondrocyte cell lines, to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic developers and research laboratories a streamlined, one-stop channel for IVD raw-material procurement. For extracellular matrix and cartilage biology studies, we provide optimized antibodies like this MATN4 polyclonal, along with validated antibody pairs, blocking reagents, and rare-target sourcing. Let CamelBio be your trusted partner from concept to clinic.