Anti-MATK Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P42679

Core Product Specifications and Parameters

Biological Background: MATK Function and Localization

• Megakaryocyte-associated tyrosine-protein kinase (MATK), also known as CHK, CTK, HYL, or hematopoietic consensus tyrosine-lacking kinase, is a non-receptor tyrosine kinase encoded by the MATK gene.
• MATK plays a significant role in signal transduction of hematopoietic cells by regulating SRC-family kinase activity; it phosphorylates the C-terminal regulatory tyrosine residue of SRC-family members, which acts as a negative regulatory site, and may inhibit T-cell proliferation.
• Subcellularly, MATK is found in the cytoplasm and associated with the membrane.
• The protein is expressed in various myeloid cell lines and detected in brain and lung tissues.
• MATK belongs to the protein kinase superfamily and contains SH2 and SH3 domains, an ATP-binding site, and is involved in tyrosine phosphorylation; it undergoes alternative splicing and is a reference proteome member.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 352-466) of human MATK, suggesting that this antibody detects the full-length protein and potentially C-terminal isoforms.
• For Western blot (WB), the predicted molecular weight is ~56kDa; consider using appropriate protein ladders and validating band specificity with positive control lysates from human, mouse, or rat cells expressing MATK.
• In immunohistochemistry (IHC-P), ensure proper antigen retrieval and validate staining in known positive tissues such as brain or lung sections; include isotype controls.
• For ELISA, the antibody can be used for detection or capture formats; optimize coating and detection conditions with recombinant MATK protein or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions with a comprehensive portfolio of IVD raw materials. This anti-MATK antibody is well-suited for studies in signal transduction, kinase regulation, and hematopoietic cell biology. Beyond this product, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing services for rare targets—ensuring you have the critical reagents for your assay development and research needs.