Anti-MAT2A Rabbit Monoclonal Antibody for WB, ELISA - P31153

Core Product Specifications and Parameters

Biological Background: MAT2A Function and Localization

• MAT2A encodes S-adenosylmethionine synthase isoform type-2, also known as methionine adenosyltransferase II (MAT-II). It is characterized by alternative splicing and undergoes post-translational modifications including acetylation, phosphorylation, and Ubl conjugation.
• The enzyme catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP in a two-step reaction: first, AdoMet and triphosphate are formed, followed by hydrolysis of the triphosphate.
• MAT2A plays a central role in one-carbon metabolism by supplying AdoMet, the universal methyl donor required for numerous methylation reactions in the cell.
• It functions as an ATP- and nucleotide-binding enzyme, with activity dependent on magnesium and potassium ions.
• Expression of MAT2A has been detected in kidney tissue, indicating a role in methylation processes in this organ.
• MAT2A is the catalytic subunit of the MAT-II isozyme and belongs to the AdoMet synthetase family, distinct from the MAT-I/III isoforms encoded by MAT1A.
• Structural data (3D-structure keyword) have revealed the enzyme's catalytic mechanism, contributing to a deeper understanding of its function and potential as a therapeutic target.

Experimental Guidance and Technical Tips

• The immunogen for this antibody is a synthetic peptide from the N-terminal region (amino acids 1-100) of human MAT2A. This design may provide specific recognition of the target protein.
• For Western blotting (WB), a band at approximately 44 kDa is expected, consistent with the calculated molecular weight of 43.7 kDa. Validate in the relevant sample system.
• In ELISA applications, the antibody can be used for antigen detection; consider optimal coating and detection conditions.
• Cross-reactivity has been indicated for human, mouse, and rat. If working with other species, sequence homology should be examined and reactivity verified experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. This MAT2A Rabbit mAb targets a key enzyme in one-carbon metabolism and methylation pathways, making it valuable for research in cancer, epigenetics, and metabolic disorders. As a one-stop sourcing partner, CamelBio can also assist with rare target raw-material procurement and technical consulting.