Anti-MAT2A Polyclonal Antibody for WB, ELISA - P31153

Core Product Specifications and Parameters

Biological Background: MAT2A Function and Localization

• MAT2A encodes S-adenosylmethionine synthase isoform type-2 (MAT2A), also known as methionine adenosyltransferase 2 (MAT 2) or MAT-II.
• It catalyzes the two-step formation of S-adenosylmethionine (AdoMet) from methionine and ATP, a critical methyl donor in one-carbon metabolism.
• The enzyme is detected in kidney, suggesting a role in tissue-specific AdoMet synthesis and methylation dynamics.
• MAT2A belongs to the transferase family and requires metal cofactors such as magnesium and potassium for activity; it also binds ATP through a nucleotide-binding domain.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin conjugation, indicating extensive regulatory control.
• Alternative splicing yields multiple isoforms, potentially diversifying subcellular localization or functional specialization.
• Associated keywords: One-carbon metabolism, Transferase, Metal-binding, Nucleotide-binding, Acetylation, Phosphoprotein, Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein spanning the N-terminal region (aa 1–395), making this antibody suitable for detecting full-length MAT2A in Western blot (predicted molecular weight ~44 kDa).
• Validated cross-reactivity across human, mouse, and rat ensures broad model system applicability; confirm detection in your specific tissue or cell lysate.
• For ELISA, consider using recombinant MAT2A protein or a peptide derived from the immunogen sequence as a control antigen to validate specificity and sensitivity.
• Optimize antibody dilutions empirically in the relevant sample matrix, and include appropriate negative controls to confirm signal authenticity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material sourcing, offering a one-stop bridge for diagnostic manufacturers and research laboratories. With this anti-MAT2A polyclonal antibody targeting a central one-carbon metabolism enzyme, researchers can access validated pair reagents, bulk ancillary materials, and technical consulting to accelerate assay development for methylation-related biomarkers.