Anti-Mast Cell Tryptase (TPSB2) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q15661

Core Product Specifications and Parameters

Biological Background: Tryptase alpha/beta-1 Function and Localization

• Tryptase alpha/beta-1 (UniProt: Q15661), also known as tryptase I or tryptase alpha-1, is a serine protease encoded by the TPSAB1 gene. It shares high sequence homology with TPSB2 (tryptase beta-2).
• It functions as the major neutral protease in mast cell secretory granules and is released during mast cell degranulation, playing a key role in inflammatory and allergic responses.
• The protease is implicated in innate immunity through the cleavage of various substrates. Isoform 2 (also referred to as tryptase beta-1) exhibits differential substrate specificity, more efficiently cleaving large substrates such as fibronectin but less efficiently processing small substrates compared to isoform 1.
  Related references:
  PMID:18854315
• Tryptase alpha/beta-1 is secreted from cells, consistent with its role as an extracellular mediator after mast cell activation.
• Isoforms display distinct tissue distributions: both are found in lung, stomach, spleen, heart, and skin (with isoform 1 predominating), while isoform 2 is additionally detected in aorta, spleen, and breast tumors, particularly in endothelial cells of perivascular regions and tumor vasculature, with minimal mast cell expression at the protein level.
• The protein is post-translationally modified by glycosylation and disulfide bond formation, and is synthesized as a zymogen with a cleavable signal peptide. It is subject to alternative splicing, yielding multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 30–275 of human TPSB2 (mature tryptase without the signal peptide), suggesting the antibody may recognize both pro- and active forms. For WB, the predicted molecular weight is approximately 31 kDa, but glycosylation can increase the apparent mass; consider using deglycosylation enzymes if a shift is observed.
• In immunohistochemistry (IHC-P), robust staining is expected in mast cell-rich tissues such as skin, lung, and gastrointestinal mucosa. Antigen retrieval methods (e.g., heat-induced epitope retrieval) are recommended, and appropriate isotype controls should be included.
• For ELISA applications, the antibody may be used for capture or detection; titrate the antibody and validate performance with recombinant tryptase standards or mast cell lysates to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-materials partner, CamelBio provides comprehensive access to validated antibodies, antibody pairs, and recombinant proteins for diagnostic assay development. Our portfolio includes rigorously screened monoclonal and polyclonal antibodies against mast cell targets, such as tryptase, enabling researchers and manufacturers to accelerate biomarker discovery and assay prototyping. We support bulk procurement of ancillary reagents and offer rare target sourcing services—connecting your projects with high-quality raw materials from R&D through clinical application. For mast cell research and beyond, CamelBio streamlines your supply chain with technical consultation and reliable lot-to-lot consistency.