Anti-Mast Cell Chymase (CMA1) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P23946

Core Product Specifications and Parameters

Biological Background: Mast Cell Chymase Function and Localization

• Mast Cell Chymase (CMA1), also known as alpha-chymase or mast cell protease I, is a serine protease that serves as the major secreted enzyme of mast cells.
• Its primary functions include generation of vasoactive peptides, degradation of extracellular matrix components, and modulation of glandular secretion.
• The CMA1 gene belongs to the peptidase S1 family and encodes a zymogen with a signal peptide that is processed into the active protease.
• The protein localizes to cytoplasmic granules in mast cells and is secreted upon degranulation.
• CMA1 is predominantly expressed in mast cells of the lung, heart, skin, and placenta, and has been detected in both normal skin and urticaria pigmentosa lesions.
• It undergoes alternative splicing, is glycosylated, and contains critical disulfide bonds for structural stability; its three-dimensional structure has been solved.
• The mature chymase has a molecular weight of approximately 27.3 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning residues 148-247, suggesting reactivity toward the mature, processed form of chymase.
• For Western blot (WB), a band around 27 kDa is expected; consider optimizing blocking and antibody dilution to reduce non-specific binding.
• In immunohistochemistry (IHC-P), mast cell-rich tissues such as skin or lung are suitable; validate detection in your specific tissue type and fixation conditions.
• For ELISA, the antibody may be used as a capture or detection reagent; cross-reactivity with mouse and rat chymase should be confirmed experimentally in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing bridge for IVD raw materials, connecting diagnostic developers with high-quality reagents like this mast cell chymase antibody for research in allergy, inflammation, and mast cell biology. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your assay development from concept to clinic.