Anti-MARVELD2 Rabbit Polyclonal Antibody for WB, ELISA - Q8N4S9

Core Product Specifications and Parameters

Biological Background: MARVELD2 Function and Localization

• MARVEL domain-containing protein 2 (tricellulin) is a transmembrane protein encoded by the MARVELD2 gene; alternative splicing produces multiple isoforms.
• It plays a critical role in the formation of tricellular tight junctions, maintaining epithelial barrier integrity by sealing contacts where three cells meet.
• MARVELD2 is required for normal hearing: it separates endolymphatic and perilymphatic spaces in the organ of Corti and is essential for hair cell survival. Related references: PMID:17186462
• The protein localizes to the cell membrane and specifically to tight junctions, consistent with its barrier function.
• Post-translational modifications include isopeptide bond formation, ubiquitin-like conjugation, and phosphorylation, suggesting regulatory mechanisms.
• Keywords associated with MARVELD2 include deafness, non-syndromic deafness, and coiled-coil domain, reflecting its clinical and structural features.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved internal region (aa 200-300); verify cross-reactivity in your target species prior to large-scale experiments.
• For western blotting, a major band is expected at approximately 64 kDa under reducing conditions. Additional bands may appear due to isoforms, post-translational modifications, or endogenous cleavage.
• When using this polyclonal antibody in ELISA, consider potential lot-to-lot variability and validate consistency in your assay conditions.
• Since MARVELD2 is a tight junction protein, extraction protocols for membrane fractions are recommended to improve detection sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams with reliable access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for tight junction and hearing loss research. If your project requires rare or custom raw materials, our sourcing network can help secure what you need. Connect with CamelBio to bridge the gap from discovery to clinical application efficiently.