Anti-MARK3 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P27448

Core Product Specifications and Parameters

Biological Background: MARK3 Function and Localization

• MARK3, also known as C-TAK1 (cTAK1), Par-1a (PAR1A), KP78, VIPB, or EMK-2, is a serine/threonine-protein kinase encoded by the MARK3 gene. Alternative splicing generates multiple isoforms of the protein.
• MARK3 phosphorylates microtubule-associated proteins MAP2 and MAP4, and specifically phosphorylates MAPT/TAU on serine residues, contributing to microtubule dynamics regulation (PubMed:23666762). Related references: PMID:23666762
• It phosphorylates CDC25C on Ser-216, leading to 14-3-3 binding and cell cycle arrest (PubMed:12941695). Related references: PMID:12941695
• MARK3 regulates the localization and activity of histone deacetylases (e.g., HDAC7) and transcription factor MITF by phosphorylating them, promoting 14-3-3 binding and nuclear export or cytosolic retention, respectively (PubMed:16980613, PubMed:16822840). Related references: PMID:16980613 PMID:16822840
• It negatively regulates the Hippo signaling pathway by cooperating with DLG5 to inhibit STK3/MST2 kinase activity, thereby blocking LATS1 phosphorylation (PubMed:28087714). Related references: PMID:28087714
• Subcellular localization includes the cell membrane, cytoplasmic side, dendrites, and cytoplasm, consistent with its roles in membrane-associated signaling and cytoskeletal regulation.
• It is ubiquitously expressed, indicating broad functional importance across tissue types.
• The protein is an ATP-binding phosphoprotein with kinase activity, and is classified under serine/threonine-protein kinase transferase family.

Experimental Guidance and Technical Tips

• The antibody was raised against an N-terminal peptide (aa 1-100) of human MARK3, a region highly conserved across species, supporting its cross-reactivity with mouse and rat MARK3 as indicated.
• For Western blotting, expect a band near 84 kDa corresponding to full-length MARK3; however, alternative splice variants may produce additional bands. Validate with appropriate positive and negative controls.
• In IHC-P, MARK3 localizes to the cell membrane, dendrites, and cytoplasm. Consider using antigen retrieval and validate staining patterns against known expression data.
• For ELISA applications, the antibody can be used as a capture or detection reagent; optimize coating concentrations and blocking conditions for your specific assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for IVD raw materials, offering validated antibody pairs, bulk ancillary reagents, and targeted sourcing for rare targets. For researchers and manufacturers focusing on kinase signaling, cell cycle regulation, or neurodegenerative disease research involving MARK3, we connect you with optimized polyclonal antibodies and technical support to accelerate assay development. Explore our full portfolio to streamline your sourcing from concept to clinical application.