Anti-MARK2 Rabbit Monoclonal Antibody for WB and ELISA - Q7KZI7

Core Product Specifications and Parameters

Biological Background: MARK2 Function and Localization

• MARK2 (also known as EMK-1, PAR-1, Par1b) is a serine/threonine-protein kinase that phosphorylates the microtubule-associated protein TAU (MAPT) at KXGS motifs, promoting TAU detachment from microtubules and microtubule disassembly, thereby regulating cell polarity.
  Related references: PMID:23666762
• It plays a broad role in cell polarity by phosphorylating additional microtubule-associated proteins MAP2 and MAP4 at similar motifs, leading to their dissociation from the cytoskeleton.
• MARK2 regulates neuronal polarization and migration through phosphorylation of DCX and KIF13B, influencing 14-3-3 protein interactions and microtubule-dependent transport processes.
• It modulates Wnt signaling by phosphorylating disheveled proteins (DVL1, DVL2, DVL3), acting as a positive regulator of the pathway.
• Through phosphorylation of HDAC7, MARK2 promotes its interaction with 14-3-3 and nuclear export, thereby regulating histone deacetylase function.
• In response to ER stress or starvation-induced autophagy, MARK2 phosphorylates UBAC2 at Ser-223, facilitating ER-phagy.
  Related references: PMID:39284914
• Subcellular localization includes the cell membrane, cytoplasm, lateral cell membrane, cytoskeleton, and dendritic projections, consistent with its roles in polarity and trafficking.
• High expression in heart, brain, skeletal muscle, and pancreas, with lower expression in lung, liver, and kidney, aligns with its physiological functions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (aa 689-788) of human MARK2, which shares high sequence identity with mouse and rat orthologs, supporting the observed cross-reactivity.
• For Western blot applications, the predicted molecular weight is approximately 88 kDa; we recommend using positive control lysates from cell lines with known MARK2 expression (e.g., HeLa, HEK293) and optimizing antibody dilution.
• In ELISA assays, consider using recombinant MARK2 protein or the immunogen peptide as the capture antigen; validate assay conditions with a standard curve to ensure linearity and sensitivity.
• Due to its role in cell polarity and microtubule dynamics, additional functional validation in immunofluorescence or immunohistochemistry may require fixation/permeabilization optimization to preserve the native localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes this high-quality anti-MARK2 rabbit monoclonal antibody for cell polarity and neurobiology research, alongside validated antibody pairs, bulk ancillary reagents, and custom antibody development services. From rare target sourcing to large-scale production, we streamline your procurement process to accelerate assay development.