Anti-MARK1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9P0L2

Core Product Specifications and Parameters

Biological Background: MARK1 Function and Localization

• MARK1 (MAP/microtubule affinity-regulating kinase 1) is a serine/threonine-protein kinase that belongs to the MARK family, which also includes MARK2, MARK3, and MARK4.
• It phosphorylates microtubule-associated proteins such as MAP2, MAP4, and MAPT/TAU at KXGS motifs, leading to their detachment from microtubules and contributing to microtubule disassembly. Related references: PMID:23666762
• MARK1 plays a critical role in regulating cell polarity and neuronal migration by modulating the cytoskeleton, and it phosphorylates DCX (doublecortin) to influence neuronal migration.
• The kinase acts as a positive regulator of the Wnt signaling pathway, likely through phosphorylation of dishevelled proteins (DVL1, DVL2, DVL3).
• Alternative splicing produces multiple isoforms of MARK1, which may have distinct functional roles.
• Subcellularly, MARK1 localizes to the cell membrane, cytoplasm, cytoskeleton, and dendrites, consistent with its role in cell polarity and microtubule dynamics.
• It is highly expressed in the heart, skeletal muscle, brain, fetal brain, and fetal kidney, indicating tissue-specific functions in these organs.
• MARK1 requires magnesium for its kinase activity and ATP binding, and it is regulated by phosphorylation and lipid binding.

Experimental Guidance and Technical Tips

• For Western blotting, anticipate a band near 89 kDa; validate signal using lysates from tissues with high MARK1 expression such as brain or heart.
• In IHC-P, paraffin-embedded human or mouse brain sections may yield specific staining; optimize antigen retrieval conditions and confirm with appropriate controls.
• The polyclonal nature of this antibody may enhance ELISA sensitivity, but evaluate potential cross-reactivity with other MARK family members experimentally.
• Consider using the immunogen sequence (amino acids 560–700) to assess antibody specificity, particularly if full-length protein detection varies.
• When studying MARK1 phosphorylation-dependent signaling, include phosphatase inhibitors in lysis and assay buffers.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs streamlined access to validated IVD raw materials like this anti-MARK1 polyclonal antibody, supporting projects in kinase signaling and neuroscience. Our comprehensive portfolio includes optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, ensuring a reliable supply chain from concept to clinic. Partner with us to accelerate your assay development with quality raw materials and expert technical support.