Anti-MARCO Polyclonal Antibody for WB, IF-P, ELISA - Q9UEW3

Core Product Specifications and Parameters

Biological Background: MARCO Function and Localization

• Macrophage receptor MARCO (also known as SR-A6, SCARA2) is a member of the scavenger receptor class A family characterized by a collagenous structure.
• Functions as a pattern recognition receptor (PRR) that binds both Gram-positive and Gram-negative bacteria, playing a key role in innate immunity (PubMed:9468508). Related references: PMID:9468508
• Binds the secretoglobin SCGB3A2, which may modulate immune responses (PubMed:12847263). Related references: PMID:12847263
• Also involved in binding of unopsonized particles by alveolar macrophages (By similarity).
• Localizes to the cell membrane as a transmembrane receptor.
• Expressed predominantly in tissue macrophages, including alveolar macrophages, Kupffer cells of the liver, and macrophages in thymus, kidney, and spleen (PubMed:9468508).
• Contains a collagen domain, disulfide bonds, and N-glycosylation sites, and exists in alternatively spliced forms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 421-520 of human MARCO, located in the C-terminal extracellular region; the resulting polyclonal antibody likely recognizes multiple epitopes.
• For Western blotting, the predicted protein weight is ~53 kDa, but the observed band may shift due to post-translational modifications such as glycosylation; optimization of reducing/non-reducing conditions may be necessary.
• For immunofluorescence (IF-P), appropriate fixation and permeabilization protocols should be tested, as MARCO is a transmembrane protein.
• For ELISA, validate antibody binding using recombinant MARCO protein or cell lysates from macrophage cell lines.
• Cross-reactivity with mouse and rat has been reported, but species-specific validation in the experimental system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials, technical services, and consulting—from concept to clinic. This anti-MARCO polyclonal antibody supports research in innate immunity, macrophage biology, and infectious disease applications. Beyond individual antibodies, we offer validated antibody pairs, optimized recombinant proteins, bulk ancillary reagents, and customized sourcing for rare targets, helping you streamline your assay development pipeline.